PXD082651-1

PXD082651 is an original dataset announced via ProteomeXchange.

Title	A lifespan multi-tissue atlas of non-canonical peptides reveals organ- and life-stage-specific proteomic remodeling in mice
Description	This study presents a lifespan multi-tissue proteomic atlas of non-canonical peptides in C57BL/6 mice. Brown adipose tissue, cerebral cortex, liver, lymph nodes, and serum were analyzed at 1, 6, 12, 18, and 24 months of age using data-independent acquisition mass spectrometry (DIA-MS). An expanded protein sequence database integrating canonical mouse proteins with open reading frames derived from intronic regions, non-coding RNAs, gene fusion-related sequences, and mouse-associated microbial proteins was used for peptide identification. A total of 985 non-canonical peptides were detected, showing pronounced tissue specificity and distinct age-associated expression patterns. The study characterizes organ- and life-stage-specific remodeling of the non-canonical proteome across mouse development, maturation, and aging, and provides an age-resolved resource for investigating non-canonical translation products beyond conventional protein annotations.
HostingRepository	PRIDE
AnnounceDate	2026-08-16
AnnouncementXML	Submission_2026-08-16_05:08:45.674.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuxin Zheng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-15 16:10:51	ID requested
⏵ 1	2026-08-16 05:08:46	announced

Dataset with its publication pending

submitter keyword: short open reading frames, DIA-MS, development, C57BL/6 mice,non-canonical peptides, lifespan, multi-tissue proteomics, aging

Xiaotong Luo
contact affiliation	Innovation Center of the Sixth Affiliated hospital, School of Life Sciences, Sun Yat-senUniversity, Guangzhou
contact email	luoxt36@mail.sysu.edu.cn
lab head
Shuxin Zheng
contact affiliation	Innovation Center of the Sixth Affiliated Hospital, School of Life Sciences, Sun Yat-Sen University, Guangzhou
contact email	zhengshx26@mail2.sysu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD082651
     1. Label: PRIDE project
     2. Name: A lifespan multi-tissue atlas of non-canonical peptides reveals organ- and life-stage-specific proteomic remodeling in mice