PXD082586-1

PXD082586 is an original dataset announced via ProteomeXchange.

Title	Reverse Transcriptase Inhibitors regulate LINE-1 expresion, modulate ORF1p protein interactions, and activate autophagy in cancer cells
Description	Long Interspersed Element-1 (LINE-1; L1) is an autonomous retrotransposon whose expression is frequently dysregulated in cancer. L1 encodes two proteins, ORF1p and ORF2p, which are essential for retrotransposition. Reverse transcriptase inhibitors (RTIs), including nucleoside (NRTIs) and non-nucleoside reverse transcriptase inhibitors (NNRTIs), have been reported to exert anti-cancer effects; however, the molecular mechanisms underlying these effects remain incompletely understood. This project investigates the cellular response to RTI treatment and its relationship with LINE-1 expression and activity. RTI treatment also promoted the association of ORF1p with proteins involved in transcriptional regulation, DNA damage, and nuclear architecture.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-16_21:33:18.005.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Omar Guillermo Rosas Bringas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	L-cysteine methyl disulfide; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-14 04:54:51	ID requested
⏵ 1	2026-08-16 21:33:18	announced

10.1101/2025.08.07.669170;

Damizia M, Baranzini M, Sciamanna I, Altieri L, Rovella P, Rosas Bringa OG, Hozeifi S, Saba LJ, Mourtzinos A, O'Shea A, Ansari MSZ, Andreola F, Cirilli R, Sbardella G, Serafino A, Schumann GG, Trisciuoglio D, LaCava J, Lavia P, Spadafora C, Reverse transcriptase inhibitors induce autophagy in a LINE-1 ORF1p-dependent manner. bioRxiv, ():(2025) [pubmed]

submitter keyword: Human

IP-MS

LINE-1

ORF1p

John LaCava
contact affiliation	University of Nebraska Medical Center
contact email	jlacava@unmc.edu
lab head
Omar Guillermo Rosas Bringas
contact affiliation	PhD
contact email	orosasbringas@rockefeller.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD082586

PRIDE project URI

• PRIDE
  1. PXD082586
     1. Label: PRIDE project
     2. Name: Reverse Transcriptase Inhibitors regulate LINE-1 expresion, modulate ORF1p protein interactions, and activate autophagy in cancer cells