PXD082463-1

PXD082463 is an original dataset announced via ProteomeXchange.

Title	AAV9-LC3B–mediated activation of autophagy alleviates hippocampal developmental injury caused by intrauterine inflammation via inhibition of the PirB/PI3K/AKT/mTOR pathway
Description	Globally, preterm birth represents a leading preventable contributor to neonatal mortality and long-term neurodevelopmental sequelae, with an annual 13.4 million infants born prematurely, and this clinical condition is commonly complicated by cognitive and motor functional deficits. Intrauterine bacterial infection and inflammation are core factors triggering preterm birth and fetal brain injury. Herein, we used a rat model of intrauterine inflammation induced by lipopolysaccharide, and an AAV9 vector–mediated LC3B overexpression system was established to evaluate its effects on offspring hippocampal development and cognitive function. Our data revealed that LC3B overexpression significantly ameliorated spatial learning and memory performance of offspring rats in the Morris water maze assay, which was supported by decreased escape latency and more frequent crossings of the previous platform position. In addition, immunohistochemistry and Western blot analyses verified that LC3B overexpression markedly attenuated the activation response of hippocampal astrocytes and microglia. Proteomic analysis revealed that LC3B overexpression significantly altered the hippocampal protein expression profile, with differentially expressed proteins enriched in autophagy- and metabolism-related pathways. Further validation showed that intrauterine LPS exposure induced abnormal upregulation of PirB protein, whereas LC3B overexpression significantly inhibited PirB expression and excessive activation of the PI3K/AKT/mTOR signaling pathway, while promoting the upregulation of autophagy-related proteins. Collectively, these findings indicate that LC3B overexpression activates autophagy by suppressing aberrant PirB expression and modulating the PI3K/AKT/mTOR pathway, thereby alleviating neuroinflammation and preserving cognitive function. Our study identifies a novel molecular target and establishes a theoretical basis for the prophylaxis and treatment of brain injury in premature neonates.
HostingRepository	iProX
AnnounceDate	2026-08-13
AnnouncementXML	Submission_2026-08-13_01:21:59.560.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bocheng Xiong
SpeciesList	scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-13 01:21:20	ID requested
⏵ 1	2026-08-13 01:22:00	announced

Dataset with its publication pending

submitter keyword: Intrauterine inflammation, Preterm birth,Autophagy, PirB

Xifei Yang
contact affiliation	Shenzhen Center for Disease Control and Prevention
contact email	xifeiyang@gmail.com
lab head
Bocheng Xiong
contact affiliation	Shenzhen Center for Disease Control and Prevention
contact email	1198696493@qq.com
dataset submitter

iProX dataset URI