PXD082410-1

PXD082410 is an original dataset announced via ProteomeXchange.

Title	Prolonged in vitro Expansion Shapes the Neuro-supportive Po-tential of Jaw Periosteum Secretomes: Implications for Secre-tome Product Quality
Description	Nerve injuries are frequent complications of complex oral and maxillofacial surgical procedures, particularly following extensive tumor resections. Secretome-based, cell-free therapies derived from mesenchymal stromal cells have emerged as promising regenerative approaches; however, robust manufacturing requires the identification of critical quality attributes (CQAs) that ensure product potency and consistency. The in-fluence of replicative senescence during in vitro expansion on the quality of jaw peri-osteum-derived mesenchymal stromal cell (JPC) secretomes has not yet been estab-lished. This study investigated whether the expansion state of JPCs affects the composition and neuro-supportive potency of their secretomes. Secretomes from four independent JPC donors were collected separately at early and late passages, pooled within each pas-sage-specific preparation, and applied to human induced pluripotent stem cell-derived neurons. Neuronal survival, neurite outgrowth, and neuronal marker expression were assessed as functional readouts. Secretome composition was characterized by quanti-tative proteomics and enzyme-linked immunosorbent assay (ELISA) of selected se-nescence-associated secretory phenotype (SASP) factors. Secretomes derived from early-passage JPCs significantly enhanced neuronal survival and neurite outgrowth, whereas late-passage secretomes displayed reduced neu-ro-supportive activity. Proteomic profiling identified a pronounced shift toward in-flammatory and stress-associated signaling, whereas performed ELISAs confirmed senescence-associated remodeling of the secretome, including increased abundance of SASP-associated factors in late-passage preparations. These findings demonstrate that prolonged in vitro expansion profoundly influences both the composition and biological potency of JPC-derived secretomes. Collectively, this study identifies the passage-associated senescence-like phenotype of JPCs as a key determinant of secretome quality and supports its consideration as a critical quality attribute for the manufacturing and standardization of JPC-derived se-cretome products. Monitoring and controlling the expansion state of JPCs may therefore be essential to ensure the consistency, potency, and clinical translation of secre-tome-based regenerative therapies.
HostingRepository	MassIVE
AnnounceDate	2026-08-11
AnnouncementXML	Submission_2026-08-11_04:19:17.305.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohamed Ali Jarboui
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Acetyl; Oxidation
Instrument	timsTOF Ultra 2

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-11 03:01:52	ID requested
⏵ 1	2026-08-11 04:19:17	announced

no publication

submitter keyword: jaw periosteum, secretome, paracrine communication, passage-associated senes-cence-like phenotype, differentiation of iPSCs to NGN2 neurons, neuroregeneration, DIA, Proteomics, Secretome, DatasetType:Proteomics

Mohamed Ali Jarboui
contact affiliation	Core Facility Medical Proteomics, University Tuebingen
contact email	mohamed-ali.jarboui@uni-tuebingen.de
lab head
Mohamed Ali Jarboui
contact affiliation	University klinikum Tuebingen
contact email	mohamed-ali.jarboui@uni-tuebingen.de
dataset submitter

MassIVE dataset URI

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