PXD082271 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Chemical profiling and in vitro cytotoxicity assessment of commercial Red Yeast Rice ingredients |
| Description | Red yeast rice (RYR) food supplements are widely used for cholesterol management due to their monacolin content, particularly monacolin K (MK), which shares the same chemical structure as lovastatin, a compound associated with several side effects. The European Food Safety Authority (EFSA) highlighted uncertainties regarding the compositional vari-ability, characterization of bioactive constituents, and safety of RYR products, concluding that a safe level of monacolin intake could not be established. In this study, twenty-seven commercial semi-processed RYR ingredients for food supplements manufacturing were investigated through a combined chemical and biological approach to characterize their composition and evaluate potential safety concerns. Targeted LC-MS/MS analyses re-vealed substantial variability in MK, monacolin hydroxy acid (MKA), and dehydromon-acolin K (DMK) levels among products. Exploratory qualitative untargeted metabolomics enabled the putative annotation of multiple secondary metabolites, including azaphilones and polyketides, highlighting differences in chemical profiles. Correlation analyses indi-cated that DMK content was associated with pigment distribution and cytotoxic responses in human hepatocellular carcinoma cells and primary human skeletal muscle cells, whereas MK and MKA showed no significant association under the tested conditions. Proteomic analyses further revealed distinct cellular responses between RYR extracts and simvastatin. These findings emphasize the importance of comprehensive chemical char-acterization and quality control strategies considering both monacolins and secondary metabolites to improve the safety assessment of RYR supplements. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-20 |
| AnnouncementXML | Submission_2026-08-20_00:59:41.083.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mattia Cicogni |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-08-06 08:12:05 | ID requested | |
| ⏵ 1 | 2026-08-20 00:59:41 | announced | |
Publication List
Keyword List
| submitter keyword: phytochemical characterization,untargeted metabolomics, proteomics, bioinformatics, secondary metabolites, food safety, red yeast rice, cytotoxicity |
Contact List
| Laura Salvini |
|---|
| contact affiliation | Toscana Life Sciences |
| contact email | l.salvini@toscanalifesciences.org |
| lab head | |
| Mattia Cicogni |
|---|
| contact affiliation | toscana life sciences |
| contact email | m.cicogni@toscanalifesciences.org |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD082271 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD082271
- Label: PRIDE project
- Name: Chemical profiling and in vitro cytotoxicity assessment of commercial Red Yeast Rice ingredients
