PXD082252-1

PXD082252 is an original dataset announced via ProteomeXchange.

Title	Cost-Effective DiskTip Spatially Resolved Micro-Proteomics Uncovers Region-Specific Proteome Changes and IgSF CAM-RHOA-MAPK Cascade in LPS Neuroinflammation
Description	We developed a low‑cost SCX‑membrane‑based DiskTip microproteomics workflow, integrated with LCM and DIA‑MS, to profile six brain regions in LPS‑induced neuroinflammation. We uncovered a molecular boundary between hippocampal CA1 and CA3 with opposing MAPK activity, and proposed the Spatial Heterogeneity Index (SHI) to quantify regional variation. The IgSF CAM–RHOA–MAPK axis may drive region‑selective lesions, providing new insights and targets for neuroinflammation.
HostingRepository	iProX
AnnounceDate	2026-08-05
AnnouncementXML	Submission_2026-08-06_00:55:00.333.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuechun Wu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-06 00:54:23	ID requested
⏵ 1	2026-08-06 00:55:00	announced

Dataset with its publication pending

submitter keyword: DiskTip, Microproteomics, Laser Capture Microdissection, LC-MS/MS

Dezhi Kong
contact affiliation	Institute of Integrative Medicine, Hebei Medical University, Key Laboratory of Tranquilizing TCM, Hebei Provincial Administration of Traditional Chinese Medicine
contact email	kongdezhi@hebmu.edu.cn
lab head
Xuechun Wu
contact affiliation	Research Ward, the Second Hospital of Hebei Medical University
contact email	2066730155@qq.com
dataset submitter

iProX dataset URI