PXD082190-1

PXD082190 is an original dataset announced via ProteomeXchange.

Title	Spermidine attenuates copper overload-induced cuproptosis in porcine granulosa cells through the miR-194a-5p/DLAT axis
Description	Excess copper disrupts mitochondrial metabolism by triggering cuproptosis, a regulated cell-death program characterised by the aggregation of lipoylated tricarboxylic acid-cycle proteins and the loss of Fe-S cluster proteins. This mitochondrial injury may be particularly consequential in ovarian granulosa cells, whose energy metabolism and biosynthetic activity sustain follicular growth. Spermidine supports mitochondrial and metabolic homeostasis, but whether it protects granulosa cells from copper overload by restraining cuproptosis remains unclear. Here, we investigated whether spermidine limits copper-overload-induced follicular injury through the miR-194a-5p/dihydrolipoamide S-acetyltransferase (DLAT) axis. Porcine atretic follicles showed increased expression of the copper transporter solute carrier family 31 member 1 (SLC31A1) and elevated copper levels in granulosa cells and follicular fluid. These changes were accompanied by reduced levels of FDX1, LIAS, DLAT, ACO2 and POLD1, diminished lipoate-associated signals and increased DLAT oligomerization. In cultured granulosa cells, CuSO₄ induced intracellular copper accumulation, mitochondrial dysfunction and cuproptosis-associated injury, which was preferentially attenuated by ammonium tetrathiomolybdate. Spermidine supplementation attenuated copper-overload-induced ovarian injury in mice and protected cultured granulosa cells from mitochondrial dysfunction, whereas polyamine depletion aggravated the loss of Fe-S cluster and lipoylated proteins. Mechanistically, spermidine reduced miR-194a-5p abundance, restored DLAT expression and preserved pyruvate dehydrogenase activity, ATP production, NDUFS1 concentration and ACO2 activity. Together, these findings support a spermidine/miR-194a-5p/DLAT axis that restrains copper-overload-induced cuproptosis-associated injury in granulosa cells and may link copper stress to follicular atresia.
HostingRepository	iProX
AnnounceDate	2026-08-04
AnnouncementXML	Submission_2026-08-05_01:28:03.419.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Wang
SpeciesList	scientific name: Sus scrofa domesticus; NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-05 01:27:38	ID requested
⏵ 1	2026-08-05 01:28:03	announced

Dataset with its publication pending

submitter keyword: copper, cuproptosis, spermidine, miR-194a-5p, DLAT, follicular atresia

Bo Kang
contact affiliation	Sichuan Agricultural University
contact email	bokang@sicau.edu.cn
lab head
Xin Wang
contact affiliation	Sichuan Agricultural University
contact email	xinwang@stu.sicau.edu.cn
dataset submitter

iProX dataset URI