⮝ Full datasets listing
PXD082104-1
PXD082104 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Exploring Protein Structure and Interactions via Electrophoto-XL-MS with Phosphate-Enrichable Cross-linker |
| Description | Chemical cross-linking mass spectrometry (XL-MS) is a powerful research tool for exploring protein-protein interactions and their three-dimensional structures. To further increase cross-link coverage, a novel, methyurazole-derived, enrichable trifunctional cross-linker—phospho bismethylurazole (pBMT) was developed. The phosphate group, a small affinity tag, successfully separates cross-linked peptides from high-abundance noncross-linked peptides. The cross-linking strategy was developed to target multiple amino acid types ( i.e., tyrosine and histidine) under electrochemical and photochemical conditions without additional side reactions. This method was applied to cross-linking peptides, proteins, protein complexes, and cell lysates. Analysis of cross-linking results indicates that the pBMT-based cross-linking strategy provides abundant and accurate information for facilitating protein structure elucidation. Altogether, pBMT provides a novel chemical biology tool and innovative thought for the in-depth exploration of dynamic interactions between proteins |
| HostingRepository | iProX |
| AnnounceDate | 2026-08-03 |
| AnnouncementXML | Submission_2026-08-03_17:58:14.360.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhonglin Wei |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-08-03 17:57:44 | ID requested | |
| ⏵ 1 | 2026-08-03 17:58:15 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Phosphate-Enrichable, XL-MS |
Contact List
| Zhonglin Wei | |
|---|---|
| contact affiliation | Jilin University |
| contact email | zlwei@jlu.edu.cn |
| lab head | |
| Zhonglin Wei | |
| contact affiliation | Jilin University |
| contact email | zlwei@jlu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
