PXD082061-1

PXD082061 is an original dataset announced via ProteomeXchange.

Title	Comparative membrane proteomic profiling of macrophages harboring intracellular methicillin-resistant Staphylococcus aureus
Description	Chronic septic arthritis caused by methicillin-resistant Staphylococcus aureus (MRSA) is characterized by persistent infection, dysregulated host immunity and progressive joint destruction. However, the membrane-associated proteomic features that distinguish host cells harboring intracellular MRSA from neighboring cells exposed to the same infectious microenvironment remain incompletely understood. In this study, a murine model of chronic septic arthritis was established using GFP-expressing MRSA. Cells isolated from infected joint tissues were separated by fluorescence-activated cell sorting into GFP-negative and GFP-positive populations. The GFP-negative population represented cells from the infected joint microenvironment without detectable intracellular GFP signal, whereas the GFP-positive population represented cells containing detectable intracellular GFP-MRSA. Membrane-enriched protein fractions from both populations were isolated and subjected to liquid chromatography–tandem mass spectrometry for comparative membrane proteomic profiling. Differentially abundant membrane-associated proteins and biological pathways related to transmembrane transport, redox homeostasis, innate immune responses, phagosome–lysosome function, protein homeostasis and host–pathogen interactions were characterized. Particular attention was given to membrane transporters and immune-regulatory proteins associated with intracellular bacterial persistence. This dataset provides an in vivo membrane proteomic resource for identifying intracellular MRSA-associated remodeling of host-cell membrane protein networks during chronic septic arthritis.
HostingRepository	iProX
AnnounceDate	2026-08-02
AnnouncementXML	Submission_2026-08-03_00:38:06.568.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zixuan Ou
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-03 00:37:31	ID requested
⏵ 1	2026-08-03 00:38:07	announced

Dataset with its publication pending

submitter keyword: MRSA, Staphylococcus aureus, macrophage, intracellular infection, membrane proteomics, SLC7A11, redox homeostasis, immune evasion

Cao Yang
contact affiliation	Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact email	caoyangunion@hust.edu.cn
lab head
Zixuan Ou
contact affiliation	Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact email	1206546835@qq.com
dataset submitter

iProX dataset URI