PXD082028-1

PXD082028 is an original dataset announced via ProteomeXchange.

Title	Amino acid conjugation detoxifies fusaric acid in Streptomyces sp. ATMOS43
Description	Plant-associated streptomycetes are persistent in plant microbiomes, yet how they cope with pathogen-derived toxins remains poorly understood. Fusarium species produce fusaric acid (FA), a mycotoxin that contributes to virulence and perturbs plant-associated microbial communities. Here, we show that the Arabidopsis endophyte Streptomyces sp. ATMOS43 exhibits characteristic tolerance to FA. Metabolomics analysis revealed the formation of single amino acid and dipeptidyl conjugates of FA, including FA-Ser, a major conjugate that lacked detectable toxicity in bacterial and plant assays. Co-cultivation experiments showed that FA detoxification by ATMOS43 restores growth of sensitive streptomycetes in the presence of FA. This activity is enriched among plant-associated isolates, suggesting adaptation to toxin-rich environments. Proteomics and physiological analyses further indicate FA toxicity involves zinc chelation, which is abolished upon conjugation to FA-Ser, although additional cellular targets may also contribute. Together, our findings show that streptomycetes can protect plants not only by inhibiting pathogens themselves, but also by neutralizing pathogen-derived toxins.
HostingRepository	PRIDE
AnnounceDate	2026-08-03
AnnouncementXML	Submission_2026-08-02_23:40:32.365.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chao DU
SpeciesList	scientific name: Streptomyces sp.; NCBI TaxID: NEWT:1931;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-02 15:37:10	ID requested
⏵ 1	2026-08-02 23:40:33	announced

Dataset with its publication pending

submitter keyword: DIA,Streptomyces, fussaric acid, timsTOF, amino acid conjugation

Gilles van Wezel
contact affiliation	Institute of Biology Leiden, Leiden University, the Netherlands
contact email	g.wezel@biology.leidenuniv.nl
lab head
Chao DU
contact affiliation	Leiden University
contact email	c.du@biology.leidenuniv.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD082028
     1. Label: PRIDE project
     2. Name: Amino acid conjugation detoxifies fusaric acid in Streptomyces sp. ATMOS43