PXD081930 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mechanistic basis for the spatiotemporal assembly of the Bloom Syndrome complex on ultrafine DNA bridges |
| Description | The BTRR (BLM/TOP3A/RMI1/RMI2) complex resolves DNA replication and recombination intermediates, as well as mitotic DNA intertwinements, yet how its diverse activities are regulated remains unclear. Here, we identify key determinants of stable assembly of the BTRR complex on ultrafine DNA bridges (UFBs). We reveal that the RMI1-RMI2 subcomplex acts as a molecular reader of hydrophobic-rich short linear motifs (SLiMs) embedded within PICH and FANCM, thereby providing a conserved mechanism for engaging distinct Superfamily2 DNA translocases during chromosome segregation and DNA repair. During UFB resolution, SLiM binding stabilises the PICH-BTRR complex assembly and supports essential interactions among BLM, PICH, and DNA. Persistent PICH-BTRR complex association post-mitosis would compromise interphase DNA damage responses and is prevented by active PICH nuclear exclusion. Our findings define the molecular basis of UFB-binding complex assembly and highlight its spatiotemporal control required to specialise BTRR complex functions.
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| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-30 |
| AnnouncementXML | Submission_2026-07-30_10:04:26.499.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Graeme Benstead-Hume |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-07-30 08:38:44 | ID requested | |
| ⏵ 1 | 2026-07-30 10:04:26 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Bloom Syndrome complex, ultrafine DNA bridges |
Contact List
| Jyoti Choudhary |
|---|
| contact affiliation | Functional Proteomics, ICR |
| contact email | jyoti.choudhary@icr.ac.uk |
| lab head | |
| Graeme Benstead-Hume |
|---|
| contact affiliation | ICR |
| contact email | ghume@icr.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD081930 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD081930
- Label: PRIDE project
- Name: Mechanistic basis for the spatiotemporal assembly of the Bloom Syndrome complex on ultrafine DNA bridges
