PXD081924-1

PXD081924 is an original dataset announced via ProteomeXchange.

Title	The PP1 phosphatase complex coordinates pre-mRNA 3’end processing and termination of RNA polymerase II transcription
Description	Native affinity purifications of V5-tagged Symplekin (Pta1) from Schizosaccharomyces pombe were analysed by label-free quantitative mass spectrometry to determine how the DPR motif of Symplekin contributes to assembly of the cleavage and polyadenylation (CPA/CPF) machinery with the PP1-PNUTS-WDR82 (PPW) phosphatase complex. Three samples were compared: an untagged mock control, wild-type Symplekin, and a Symplekin DPR-AAA motif mutant. Wild-type Symplekin co-purified with the entire CPF complex including PNUTS, WDR82 and PP1, whereas the DPR-AAA mutant retained interactions with the remainder of CPF but lost association with the PPW complex, demonstrating that the DPR motif mediates recruitment of the PP1 phosphatase module to the 3' end processing machinery.
HostingRepository	PRIDE
AnnounceDate	2026-07-31
AnnouncementXML	Submission_2026-07-31_01:53:31.805.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luke Slade
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: NEWT:4896;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-30 07:10:31	ID requested
⏵ 1	2026-07-31 01:53:32	announced

Dataset with its publication pending

submitter keyword: PNUTS, affinity purification,Schizosaccharomyces pombe, WDR82, label-free quantification, cleavage and polyadenylation, Symplekin, Pta1, PP1

Lidia Vasilieva
contact affiliation	University of Oxford, Department of Biochemistry; University of Sussex, School of Life Sciences
contact email	lidia.vasilieva@bioch.ox.ac.uk
lab head
Luke Slade
contact affiliation	University of Oxford
contact email	luke.slade@bioch.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD081924
     1. Label: PRIDE project
     2. Name: The PP1 phosphatase complex coordinates pre-mRNA 3’end processing and termination of RNA polymerase II transcription