PXD081768-1

PXD081768 is an original dataset announced via ProteomeXchange.

Title	Long-read RNA sequencing reveals an isoform switching pat-2 tern in healthy microglial cell lines exposed to radiotherapy
Description	Radiotherapy (RT) is a fundamental treatment for brain tumors, but often leads to long-term neurotoxicity, partly caused by microglial dysfunction. In this study, we used long-read RNA sequencing (LR-RNA-seq) to map the isoform-specific transcriptome of human microglial HMC3 cells after RT exposure. HMC3 human microglial cell line was treated with standard radiotherapy and, after RNA extraction and library preparation, LR-RNA-seq was performed. Further bioinformatic analyses allowed to investigate on the isoform switching pattern after radiotherapy exposure. Gene-level analysis found 591 differentially expressed genes, with positive enrichment of PI3K/AKT-related pathways and negative enrichment of translation-associated processes. Isoform-level analysis uncovered 827 significant isoform switching events across 420 genes, often involving shifts from protein-coding transcripts to non-coding variants. Functional annotation showed that most isoform switches resulted in the loss of open reading frames and protein domains, especially in ribosomal proteins and translation machinery components. Exploratory proteomic analysis identified 191 differentially abundant proteins, including a significant reduction in RPL7A, providing partial protein level support for translation-associated transcriptomic remodeling.
HostingRepository	PRIDE
AnnounceDate	2026-08-06
AnnouncementXML	Submission_2026-08-06_15:57:18.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesco Bellia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-28 04:28:58	ID requested
⏵ 1	2026-08-06 15:57:18	announced

10.3390/NCRNA12040031;

submitter keyword: Transcriptomic variants

Radiotherapy

HMC3

Orbitrap Fusion

Francesco Bellia
contact affiliation	Department of Biochemical and Biotechnological Sciences, University of Catania, Italy
contact email	francesco.bellia@unict.it
lab head
Francesco Bellia
contact affiliation	University of Catania
contact email	francesco.bellia@unict.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD081768
     1. Label: PRIDE project
     2. Name: Long-read RNA sequencing reveals an isoform switching pat-2 tern in healthy microglial cell lines exposed to radiotherapy