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PXD081724-1
PXD081724 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Allele-skewed HLA-DR Immunopeptidomes of Bordetella pertussis |
| Description | Background/Objectives: Infection with Bordetella pertussis causes whooping cough. CD4+ T cell responses depend on bacterial peptides displayed by HLA class II, yet allele- and strain-resolved maps of naturally processed B. pertussis HLA-DR ligands remain limited. We sought to define which antigens yield HLA-DR ligands in a human macrophage model and whether presentation is skewed by HLA-DR molecule and bacterial strain. Methods: THP-1-derived macrophages were pulsed with whole-cell lysates of B. pertussis vaccine/reference strain Tohama I or clinical isolate D420. HLA-DR was immunoaffinity purified; eluted peptides were identified by LC-MS/MS and assigned to HLA-DR molecules encoded by HLA-DRB1*01:01, HLA-DRB1*15:01, and HLA-DRB5*01:01. Results: We identified 63 B. pertussis peptide ligands from 29 source proteins. Presentation was skewed by HLA-DR molecule: DRB1*01:01 accounted for 37 ligands from 21 antigens, DRB1*15:01 for 22 from 7, and DRB5*01:01 for 4 from 4. Most source proteins contributed ligands primarily to one HLA-DR molecule, so an antigen that supplies peptides to one DR product need not supply peptides to another. Strain further partitioned the ligandome: 32 ligands unique to Tohama I, 12 to D420, and only 19 from 8 proteins with both lysates. Only three antigens contributed ligands to both DRB1*01:01 and DRB1*15:01; two of these, pertactin and filamentous hemagglutinin, are components of current acellular pertussis vaccines. Conclusions: B. pertussis HLA-DR ligandomes are jointly shaped by bacterial strain and HLA-DR molecule. Antigens can interact selectively with individual HLA-DR products, and peptides from a given antigen may be recovered after pulse with one strain but not another. These findings support HLA-DR- and strain-aware interpretation of class II presentation and nominate BrkA, OmpA, TcfA, and a DMT family transporter for follow-up. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-24 |
| AnnouncementXML | Submission_2026-08-24_10:02:50.239.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hooman Yari |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Bordetella pertussis; NCBI TaxID: 520; |
| ModificationList | Oxidation; Acetyl; Deamidated; Cysteinyl; Gln->pyro-Glu |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-27 13:29:37 | ID requested | |
| ⏵ 1 | 2026-08-24 10:02:50 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Bordetella pertussis, immunopeptidomics, HLA-DR, acellular pertussis vaccine, antigen presentation, peptide ligand, DatasetType:Proteomics |
Contact List
| William Hildebrand | |
|---|---|
| contact affiliation | University of Oklahoma Health Science Center |
| contact email | william-hildebrand@ouhsc.edu |
| lab head | |
| Hooman Yari | |
| contact affiliation | University of Oklahoma Health Campus |
| contact email | hooman-yari@ou.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v14/MSV000102625/ |
