PXD081720 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic profiling of THP1 wild‑type and ARG2‑knockout human monocytes under normoxic and hypoxic conditions |
| Description | Arginase 2 (ARG2) is a mitochondrial enzyme implicated in metabolic and inflammatory regulation in human monocytes. To investigate the proteomic consequences of ARG2 loss and its interaction with hypoxic stress, we performed a quantitative LC‑MS/MS analysis of THP1 wild-type (THP1WT) and ARG2‑knockout (THP1ARG2‑/‑) cells. Cells were synchronized by serum starvation and subsequently exposed for 48 hours to either normoxia (21% oxygen) or hypoxia (1% oxygen), which generates four experimental groups: THP1WT-normoxia, THP1WT‑hypoxia, THP1ARG2‑/‑‑normoxia, and THP1ARG2‑/‑‑hypoxia. Each condition was analysed in biological triplicate (12 samples total). The dataset provides a comprehensive overview of baseline proteomic differences between THP1WT and THP1ARG2‑/‑ monocytes, as well as their differential responses to hypoxic stimulation, offering a detailed resource on ARG2 related and hypoxia induced molecular changes in THP1 cells. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-28 |
| AnnouncementXML | Submission_2026-07-28_01:42:35.351.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Michael Stumpe |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-07-27 09:00:31 | ID requested | |
| ⏵ 1 | 2026-07-28 01:42:35 | announced | |
Publication List
Keyword List
| submitter keyword: THP1 |
| monocytes |
| arginase 2 |
| ARG2 knockout |
| CRISPR |
| hypoxia |
| proteomics |
| LC‑MS/MS |
| human |
| metabolic regulation |
Contact List
| Dieter Kressler |
|---|
| contact affiliation | Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland |
| contact email | dieter.kressler@unifr.ch |
| lab head | |
| Michael Stumpe |
|---|
| contact affiliation | Department of Biology,
University of Fribourg |
| contact email | michael.stumpe@unifr.ch |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD081720 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD081720
- Label: PRIDE project
- Name: Proteomic profiling of THP1 wild‑type and ARG2‑knockout human monocytes under normoxic and hypoxic conditions
