PXD081675-1

PXD081675 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic profiling of target DNA probe-associated proteins in MCF-7 cells
Description	This dataset contains label-free quantitative LC-MS/MS data from a comparative DNA affinity pull-down experiment. Two different 5′-biotinylated DNA probes were incubated with nuclear protein extracts from MCF-7 cells. Probe-associated protein complexes were enriched with streptavidin magnetic beads, eluted, digested and analyzed by nanoflow LC-MS/MS. Protein abundance in the target-probe group was compared with that in the reference-probe group to identify proteins showing preferential association with the selected DNA sequence. The submitted files include raw mass spectrometry data and processed protein identification and quantification results.
HostingRepository	iProX
AnnounceDate	2026-07-26
AnnouncementXML	Submission_2026-07-27_00:35:53.133.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinyu Miao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-27 00:35:12	ID requested
⏵ 1	2026-07-27 00:35:53	announced

Dataset with its publication pending

submitter keyword: DNA affinity pull-down, DNA-protein interaction, MCF-7 cells, nuclear proteins, label-free quantification, LC-MS/MS

Shuang Yang
contact affiliation	Laboratory of Clinical and Molecular Glycobiology, Institute of Glycome Study, Shantou University Medical College
contact email	shuangyang@stu.edu.cn
lab head
Xinyu Miao
contact affiliation	Laboratory of Clinical and Molecular Glycobiology, Institute of Glycome Study, Shantou University Medical College
contact email	miaoxinyu92@gmail.com
dataset submitter

iProX dataset URI