PXD081613-1

PXD081613 is an original dataset announced via ProteomeXchange.

Title	Post-transcriptional glucocorticoid receptor signalling synchronises circadian rhythms
Description	Circadian rhythms are driven by cellular clocks throughout the body that maintain optimal health. Glucocorticoid (GC) hormones are vital for synchronising cellular clocks, aligning them with external day/night cycles, but mechanistic understanding is incomplete. We use a glucocorticoid receptor KO/rescue approach to show residue E755 and N-terminal intrinsically disordered region are essential for GC synchronisation, whereas nuclear translocation from the cytoplasm is not. Using nascent and mature transcriptomics with (phospho)proteomics we show that, under physiological GC increases, the GR regulates a substantial proportion of gene expression post-transcriptionally. Rapid upregulation of Per1 mRNA occurs through increased RNA processing not synthesis, which drives GC synchronisation via acute increases in nascent PER1 and not PER2 protein. We find synchronisation to a single GC dose is highly heterogeneous between individual cells. Our findings provide mechanistic insight into nuclear hormone receptor signalling and establishes a cellular basis for the gradual alignment of circadian clocks by daily environmental cycles via systemic cues.
HostingRepository	PRIDE
AnnounceDate	2026-09-04
AnnouncementXML	Submission_2026-09-04_04:56:13.778.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Catarina de Matos Ferraz Franco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-24 08:43:46	ID requested
⏵ 1	2026-09-04 04:56:14	announced

Dataset with its publication pending

submitter keyword: Nuclear hormone receptor, RNA processing, circadian rhythm, glucocorticoid, clock synchronisation, entrainment, post-transcriptional regulation

John O'Neill
contact affiliation	MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, United Kingdom
contact email	oneill@mrclmb.ac.uk
lab head
Catarina de Matos Ferraz Franco
contact affiliation	MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Biomedical Campus Cambridge CB2 0QH
contact email	cffranco@mrclmb.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD081613

PRIDE project URI

• PRIDE
  1. PXD081613
     1. Label: PRIDE project
     2. Name: Post-transcriptional glucocorticoid receptor signalling synchronises circadian rhythms