PXD081598-1

PXD081598 is an original dataset announced via ProteomeXchange.

Title	Dynamic response characteristics of heart proteome of Pelopbylax nigxomaculatus during salt stress and recovery processe
Description	Salinization of freshwater ecosystems poses an escalating threat to amphibian populations worldwide, yet the molecular mechanisms underlying cardiac responses to salt stress remain poorly understood. In this study, we employed DIA-based quantitative proteomics and phosphoproteomics to systematically investigate the dynamic protein expression and phosphorylation profiles in the heart of Pelophylax nigromaculatus across low-salt, high-salt, and recovery stages. A total of 3,747 proteins and 623 phosphopeptides were identified, with phosphorylation predominantly occurring on serine residues. Kinase prediction analysis revealed that ERK2, along with AGC, TKL, TK, and STE kinase families, may serve as key upstream regulators of salt-stress signaling. Cluster analysis classified the global intersection proteins into six expression patterns, among which cluster 3 (metabolism-related) and Cluster 5 (stress-responsive) emerged as two core responsive protein populations. Functional enrichment and protein-protein interaction analyses demonstrated that high-salt stress suppressed mitochondrial aerobic respiration via down-regulation of TCA cycle enzymes, while compensatorily activating glycolysis to sustain basal energy supply—a metabolic reprogramming strategy that was largely reversed upon stress relief. Concurrently, RNA-binding proteins and stress granule assembly factors formed tight interaction networks, suggesting that RNA homeostasis maintenance serves as an auxiliary protective mechanism against salt-induced cellular damage. These findings reveal a multi-layered regulatory framework encompassing kinase-driven signal transduction, energy metabolic reprogramming, and RNA homeostasis maintenance, providing novel molecular insights into the salt tolerance and reversible repair capacity of the amphibian heart. This study not only advances our understanding of osmotic adaptation in amphibians but also establishes a foundational proteomic resource for assessing the ecological impacts of freshwater salinization on amphibian biodiversity.
HostingRepository	iProX
AnnounceDate	2026-07-24
AnnouncementXML	Submission_2026-07-24_02:14:28.573.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ningmei Wang
SpeciesList	scientific name: Pelophylax nigromaculatus; NCBI TaxID: 8409;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-24 02:13:34	ID requested
⏵ 1	2026-07-24 02:14:29	announced

Dataset with its publication pending

submitter keyword: Pelophylax nigromaculatus, salt stress, proteomics, phosphoproteomics, energy metabolism, RNA homeostasis, amphibian adaptation

Hui Wang
contact affiliation	Hebei Normal University
contact email	whui1981@163.com
lab head
Ningmei Wang
contact affiliation	Hebei Normal University
contact email	767247571@qq.com
dataset submitter

iProX dataset URI