PXD081578-1

PXD081578 is an original dataset announced via ProteomeXchange.

Title	NAGK sulfonylation data associated with "A lysis-derived PMSF and orthovanadate oxidant defines a distinct cysteine-engagement profile"
Description	The N-acetylglucosamine kinase, NAGK, was found to undergo a mobility shift on SDS-PAGE that was reliant on the presence of PMSF and orthovanadate in the lysis buffer. The mobility shift was prevented by the addition of reducing agent to the lysis buffer, so in the assay described and deposited here, we incubated human recombinant NAGK with (sample names = “plus”) or without (sample names = “minus”) PMSF and orthovanadate, ran the protein on SDS PAGE, and excised the shifted (top) and unshifted (bottom) bands for mass spectrometry-based identification of the modification. NAGK C217 was sulfonylated in the presence of PMSF and orthovanadate, indicating the two lysis buffer components generate an oxidant during cell lysis that engages cysteines. We found that NAGK is not the only target of the PMSF/orthovanadate oxidant, and that it engages a unique subset of cysteines relative to another vanadium-based oxidant.
HostingRepository	PRIDE
AnnounceDate	2026-08-08
AnnouncementXML	Submission_2026-08-07_18:41:27.486.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bethany Schaffer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-24 01:02:30	ID requested
⏵ 1	2026-08-07 18:41:27	announced

Dataset with its publication pending

submitter keyword: NAGK, PMSF, sulfonylation, orthovanadate

John Blenis
contact affiliation	Departments of Pharmacology and Biochemistry and the Sandra and Edward Meyer Cancer Center at Weill Cornell Medicine
contact email	job2064@med.cornell.edu
lab head
Bethany Schaffer
contact affiliation	Former postdoc at Weill Cornell Medicine, uploading data for paper pending acceptance from my postdoc. Currently at a startup
contact email	bethany.eleanor@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD081578
     1. Label: PRIDE project
     2. Name: NAGK sulfonylation data associated with "A lysis-derived PMSF and orthovanadate oxidant defines a distinct cysteine-engagement profile"