PXD081569-1

PXD081569 is an original dataset announced via ProteomeXchange.

Title	L8 granule regulates alternative polyadenylation
Description	Membraneless organelles participate in multiple cellular processes and layers of gene regulation. Alternative polyadenylation (APA) is emerging as a widespread mechanism for controlling gene expression by generating distinct 3′ untranslated regions (UTRs) in transcripts. However, whether specific membraneless organelles exist in mammals to regulate APA remains largely unknown. Here, we unveil a previously unidentified nuclear body, termed LENG8 granule, which functions as an active hub for APA regulation. LENG8 deletion leads to extensive 3′UTR lengthening in both cultured cells and male germ cells. LENG8 granules recruit cleavage and polyadenylation (CPA) factors and promote their local assembly, thereby favoring the utilization of proximal poly(A) sites (pPASs) for a specific group of genes. LENG8 can also compete with PABPN1 for binding to CPSF4, facilitating the assembly of the CPA complex at pPASs. Additionally, we elucidate the pivotal roles of YTHDC1-m6A landscapes in LENG8 granule formation/maintenance and target selection. The 3′UTR length of targets correlates with the formation and properties of LENG8 granules in a cell type-specific and developmental stage-dependent manner. Our findings highlight LENG8 as a key factor in 3′ end processing and uncover a hitherto unrecognized nuclear body involved in mammalian APA regulation, which broadens our understanding of membraneless organelles and their role in poly(A) site selection
HostingRepository	iProX
AnnounceDate	2026-07-23
AnnouncementXML	Submission_2026-08-13_19:28:00.598.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhicheng Wu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-23 20:06:44	ID requested
⏵ 1	2026-08-13 19:28:01	announced

Dataset with its publication pending

submitter keyword: L granule, alternative polyadenylation

Peng Dai
contact affiliation	Tongji University
contact email	daipeng@tongji.edu.cn
lab head
Zhicheng Wu
contact affiliation	Tongji University
contact email	2031507@tongji.edu.cn
dataset submitter

iProX dataset URI