PXD081555-1

PXD081555 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicles from Lactobacillus plantarum alleviate DNFB-induced atopic dermatitis in mice by reconstructing the skin barrier and inhibiting inflammation
Description	The purified EVs were resuspended in pre-cooled PBS, aliquoted into 2 mL low-adsorption centrifuge tubes, and processed in triplicate. Protein concentration was quantified using the BCA method to ensure that each sample contained at least 200 μg/mL protein. Subsequent analyses were performed by Shanghai Zhongke Xinshengsheng Biotechnology Co., Ltd., according to the following standardized procedures: (1) protein lysis followed by reduction, alkylation, and tryptic digestion; (2) separation and mass spectrometry detection using LC-MS/MS (Q-Exactive HF-X, Thermo); (3) database searching against Lactobacillus plantarum (UniProt) using MaxQuant (v1.6.17) with an FDR threshold of 1%; (4) differentially expressed proteins identification using Perseus, and functional annotations and pathway analysis via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).
HostingRepository	iProX
AnnounceDate	2026-07-21
AnnouncementXML	Submission_2026-07-23_18:44:45.866.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingying Shi
SpeciesList	scientific name: Lactobacillus reuteri; NCBI TaxID: 1598;
ModificationList	oxidation
Instrument	Trypsin

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-23 18:41:31	ID requested
⏵ 1	2026-07-23 18:44:46	announced

Dataset with its publication pending

submitter keyword: Proteomics, DIA, LC-MS/MS, Orbitrap Astral, Quantitative proteomics

Huabing Zhao
contact affiliation	Tianjin University of Science and Technology
contact email	zhaohuabing@tust.edu.cn
lab head
Qingying Shi
contact affiliation	Tianjin University of Science and Technology
contact email	shiqingying@tust.edu.cn
dataset submitter

iProX dataset URI