PXD081218-1

PXD081218 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS analysis of terminating spliceosomes
Description	In this project, we investigated how the spliceosome bound to an excised intron-lariat is remodeled and how the associated intron-lariat is debranched during the process collectively known as spliceosome termination. We used a native pulldown from a C. elegans strain carrying an endogenous tag on the spliceosome subunit PRP19 to identify factors involved in terminal splicing events. We tagged these proteins (DHX35, YJU2B, and DBR1) ectopically in human K562 cells to define the composition of terminal spliceosome intermediates by native pulldowns. We also analyzed the interactomes of several termination factors (YJU2B, DBR1, and CWF19L1), including that of the putative spliceosome quality control factor LENG1, by biotin proximity labeling in human HEK293T cells. Finally, we investigated how the terminal spliceosome bound to a debranched intron and DHX35 is remodeled. Together, our data reveal the composition and remodeling steps of terminating human spliceosomes.
HostingRepository	PRIDE
AnnounceDate	2026-08-19
AnnouncementXML	Submission_2026-08-19_04:28:26.759.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Caenorhabditis elegans; NCBI TaxID: NEWT:6239;
ModificationList	methylthiolated residue; phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480; timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-16 17:06:15	ID requested
⏵ 1	2026-08-19 04:28:27	announced

Dataset with its publication pending

submitter keyword: Human, YJU2B, CWF19L1, LENG1, C. elegans, HEK293T cells, K562 cells, DHX35, Spliceosome, PRP19, DBR1

Clemens Plaschka
contact affiliation	Research Institute of Molecular Pathology, Vienna, Austria
contact email	Clemens.plaschka@imp.ac.at
lab head
Richard Imre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD081218

PRIDE project URI

• PRIDE
  1. PXD081218
     1. Label: PRIDE project
     2. Name: LC-MS/MS analysis of terminating spliceosomes