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PXD081141-1
PXD081141 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Distinct molecular adaptations underlie convergent phenotypic responses to mannose in normal and metastatic melanocytes |
| Description | Mannose has emerged as a promising metabolic modulator capable of impairing tumor growth in phosphomannose isomerase (PMI)-deficient cancers. However, the molecular adaptations underlying the response of melanoma cells to mannose remain poorly understood. Here, we compared the phenotypic, proteomic, and phosphoproteomic responses of normal melanocytes (Melan-A) and metastatic melanoma cells (B16F10) cultured in glucose- or mannose-containing media. Both cell types exhibited similar phenotypic responses to mannose, including reduced growth, altered lactate production, and increased accumulation of acidic vesicles. Despite these shared phenotypes, proteomic and phosphoproteomic analyses revealed markedly distinct molecular adaptations. Mannose induced modest proteomic changes in normal melanocytes but extensive remodeling in metastatic melanoma cells, accompanied by profound signaling rewiring. Substrate enrichment analysis based on curated signaling interactions predicted predominantly homeostatic signaling in normal melanocytes, whereas metastatic cells exhibited enrichment of stress-responsive, survival, cell-cycle, and ubiquitin-mediated regulatory programs. Collectively, our findings demonstrate that similar phenotypic responses to mannose arise from fundamentally distinct molecular programs, highlighting cellular context as a key determinant of the adaptive response to mannose-induced metabolic stress in melanoma. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-09-01 |
| AnnouncementXML | Submission_2026-09-01_19:06:47.055.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Andre Zelanis |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Phospho; Acetyl |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-15 17:05:39 | ID requested | |
| ⏵ 1 | 2026-09-01 19:06:47 | announced |
Publication List
| de Vasconcellos Racorti N, Lima LRF, Bustos SO, Salardani M, Barcick U, de Campos CBL, Pauletti BA, Leme AFP, Chammas R, Zelanis A, Distinct molecular adaptations underlie convergent phenotypic responses to mannose in normal and metastatic melanocytes. Mol Cell Biochem, ():(2026) [pubmed] |
Keyword List
| submitter keyword: Keywords: Melanoma, phosphomannose isomerase, phosphoproteomics, mannose, cancer metabolism., DatasetType:Proteomics |
Contact List
| Andre Zelanis | |
|---|---|
| contact affiliation | Functional Proteomics Laboratory, Department of Science and Technology, Federal University of Sao Paulo, Sao Jose dos Campos, SP, Brazil |
| contact email | andre.zelanis@unifesp.br |
| lab head | |
| Andre Zelanis | |
| contact affiliation | Federal University of Sao Paulo |
| contact email | andre.zelanis@unifesp.br |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
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