PXD081064 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | N-Terminal Protein Sequencing by Nicotinic Acid Derivatization and MS Analysis |
| Description | Most MS-based workflows for N-terminal protein sequence analysis rely on the modification of primary amines at the protein N-terminus and lysine residues, in many cases through dimethylation. However, incomplete dimethyl labeling and undesired side reactions have been reported previously and were also observed in our experiments. In this study, we evaluated alternative methods for N-terminal protein derivatization. For reductive methylation, the highly toxic reducing agent sodium cyanoborohydride was successfully replaced by pyridine borane. In addition, several single-reagent labeling approaches were applied to model proteins and compared to the dimethylation workflow. Criteria for the selection of suitable reagents were completeness and selectivity of the protein derivatization reaction, the detection sensitivity for modified peptides by mass spectrometry, and the absence of undesirable side reactions. Comparative analyses demonstrated that the chemical derivatization of free amines by nicotinic acid N-hydroxysuccinimide ester is a particularly promising approach for N-terminal protein sequencing. Protocols for the nicotinoylation of proteins in solution or in gel using this reagent were optimized and successfully applied to various test proteins. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-17 |
| AnnouncementXML | Submission_2026-08-17_01:01:51.222.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Peter Gehrig |
| SpeciesList | scientific name: Equus caballus (Horse); NCBI TaxID: NEWT:9796; scientific name: Escherichia coli; NCBI TaxID: NEWT:562; scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos; Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-07-14 10:24:13 | ID requested | |
| ⏵ 1 | 2026-08-17 01:01:51 | announced | |
Publication List
Keyword List
| submitter keyword: protein sequencing, dimethylation,N-terminomics, nicotinic acid, N-terminal |
Contact List
| Paolo Nanni |
|---|
| contact affiliation | Functional Genomics Center Zurich, Switzerland |
| contact email | paolo.nanni@fgcz.uzh.ch |
| lab head | |
| Peter Gehrig |
|---|
| contact affiliation | Functional Genomics Center Zurich
University and ETH Zurich
Winterthurerstrasse 190
8057 Zurich |
| contact email | peter.gehrig@fgcz.uzh.ch |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD081064 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD081064
- Label: PRIDE project
- Name: N-Terminal Protein Sequencing by Nicotinic Acid Derivatization and MS Analysis
