PXD081058-1

PXD081058 is an original dataset announced via ProteomeXchange.

Title	Hallachrome, a marine anthraquinone, impairs cell proliferation by acting as a functional mitochondrial uncoupler in A375 melanoma cells.
Description	Backgroung: Melanoma remains one of the most lethal cancers due to its high metastatic capacity and resistance to current therapies, emphasizing the need for novel antineoplastic agents, including bioactive molecules from marine organisms, as potential sources of anticancer compound. Aims: This study aimed to investigate, for the first time, the anticancer potential of Hallachrome (HC), an anthraquinone derived from the marine polychaete Halla parthenopeia, in A375 melanoma cells. Methods: A375 melanoma cells were treated with HC in vitro to evaluate its impact on cell proliferation, migration, and morphology. Proteomic analysis was carried out to identify differentially expressed proteins in order to elucidate the biological processes modulated by the treatment. Mitochondrial morphology and function were analyzed by confocal microscopy and oxygen consumption rate, respectively, while mitochondrial membrane potential, intracellular reactive oxygen species (ROS) levels and GSH/GSSG ratio were evaluated by fluorescence-based assays. Results: HC treatment significantly reduced melanoma cell proliferation and migration in vitro, accompanied by morphological alteration and apoptosis death. Proteomic profiling revealed differential expression of several proteins involved, for example, in membrane and mitochondria organization. Moreover, HC treatment induced severe mitochondrial dysfunction, characterized by ATP depletion, mitochondrial membrane depolarization, fragmentation of the mitochondrial network, altered intracellular ROS level and GSH/GSSG ratio. Discussion: These findings suggest that HC inhibits melanoma cell survival by disrupting mitochondrial homeostasis and inducing energetic stress. Given the pivotal role of mitochondria in cancer progression, the ability of HC to interfere with mitochondrial functions supports its potential as a promising anticancer agent.
HostingRepository	PRIDE
AnnounceDate	2026-07-21
AnnouncementXML	Submission_2026-07-21_02:58:30.191.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesco Demetrio Lofaro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-14 08:50:57	ID requested
⏵ 1	2026-07-21 02:58:30	announced

10.3389/FPHAR.2026.1844144;

submitter keyword: Hallachrome (HC), natural product, marine drug, mitochondria,anthraquinones, melanoma, proteomic

Federica Boraldi
contact affiliation	Department of Life Sciences, University of Modena and Reggio Emilia (Modena, Italy)
contact email	federica.boraldi@unimore.it
lab head
Francesco Demetrio Lofaro
contact affiliation	University of Modena and Reggio Emilia
contact email	francescodemetrio.lofaro@unimore.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD081058
     1. Label: PRIDE project
     2. Name: Hallachrome, a marine anthraquinone, impairs cell proliferation by acting as a functional mitochondrial uncoupler in A375 melanoma cells.