PXD080998 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multi‑Omics Characterization of CDK4/hTERT Immortalized Ovine Satellite Cells |
| Description | Cultivated meat production requires stable, scalable muscle cell lines, yet the molecular consequences of immortalization in livestock species remain poorly characterized. In this study we establish CDK4/hTERT‑immortalized ovine satellite cells via flow‑cytometry‑based purification and dual lentiviral transduction, and perform integrated transcriptomic–proteomic profiling to define species‑specific adaptations relevant for cultivated meat applications. Label‑free quantitative proteomics was used to compare primary lamb satellite cells (PLM, passage 5) and late‑passage immortalized cells (LPILM, passage 30), revealing 960 differentially expressed proteins that collectively indicate coordinated extracellular matrix remodeling, inflammatory S100‑family activation, and erosion of canonical myogenic regulators without emergence of oncogenic signatures. Pathway and enrichment analyses (GO, KEGG) were performed to identify affected biological processes and metabolic pathways, and the proteomic dataset was further interrogated for allergenicity using AllergenOnline to support preliminary safety assessment for food use. The deposited dataset provides the full raw and processed LC‑MS/MS data, protein identifications, and quantitative values underlying all proteomics results in the manuscript, enabling reuse for comparative analyses of immortalized livestock cell lines and multi‑omics integration in cultivated meat research. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-03 |
| AnnouncementXML | Submission_2026-09-03_12:16:56.348.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | ARIAN AMIRVARESI |
| SpeciesList | scientific name: Ovis aries; NCBI TaxID: NEWT:9940; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-07-13 15:34:18 | ID requested | |
| ⏵ 1 | 2026-09-03 12:16:56 | announced | |
| 2 | 2026-09-04 01:47:19 | announced | 2026-09-04: Updated project metadata. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Cultivated meat |
| ovine muscle |
| satellite cells |
| CDK4/hTERT immortalization |
| cell line characterization |
| LC‑MS/MS |
| DIA‑PASEF |
| proteomics |
Contact List
| Reza Ovissipour |
|---|
| contact affiliation | Assistant Professor, Department of Food Science and Technology, Texas A&M University, College Station, TX 77843, USA |
| contact email | ovissi@tamu.edu |
| lab head | |
| ARIAN AMIRVARESI |
|---|
| contact affiliation | Texas A&M University |
| contact email | arianamirvaresi20@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD080998
- Label: PRIDE project
- Name: Multi‑Omics Characterization of CDK4/hTERT Immortalized Ovine Satellite Cells
