PXD080946-1

PXD080946 is an original dataset announced via ProteomeXchange.

Title	DIA-based proteomic analysis of high-yield spinosad-producing Saccharopolyspora spinosa strain ZJ-43 under malonate selection
Description	Spinosad is a potent macrocyclic lactone insecticide produced by Saccharopolyspora spinosa. However, its industrial application is significantly constrained by inherently low fermentation yields. Enhancing precursor supply (e.g., malonyl-CoA) is a key strategy for improving biosynthesis efficiency. A high-yielding parental strain was developed through ribosome engineering and NTG mutagenesis. Subsequently, three rounds of recursive genome shuffling under malonate stress yielded the superior strain ZJ-43. This strain achieved a shake-flask titer of 904.74 mg/L (5.59-fold relative to the wild-type strain). Integrated multi-omics analysis revealed that the high-yielding phenotype of ZJ-43 was associated with metabolic redistribution and attenuated TCA cycle activity, malonyl-CoA pool expansion, and an endogenous stringent response that autonomously programs the timing of secondary metabolism. These coordinated adaptations enhanced tolerance to intracellular malonyl-CoA accumulation, accelerated early growth, modified morphological differentiation, and ultimately enabled efficient spinosad overproduction. Based on these findings, we overexpressed key bottleneck genes and optimized the malonate-supplemented fermentation medium, thereby maximizing the expansion of the malonyl-CoA precursor pool in ZJ-43::fadA-accD3V235W. This engineered strain achieved a final spinosad titer of 1522.21 mg/L (9.39-fold), Overall, this work establishes and validates an integrated strategy combining composite mutagenesis, stress-based screening, multi-omics analysis, and targeted optimization, which may provide a useful framework for improving secondary metabolite production in actinomycetes.
HostingRepository	iProX
AnnounceDate	2026-07-10
AnnouncementXML	Submission_2026-08-31_01:22:16.663.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zirui Dai
SpeciesList	scientific name: Saccharopolyspora spinosa; NCBI TaxID: 60894;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-13 01:31:17	ID requested
⏵ 1	2026-08-31 01:22:17	announced

Dataset with its publication pending

submitter keyword: Saccharopolyspora spinosa, spinosad, DIA proteomics, malonate selection, ZJ-43, ppGpp, stringent response, growth-production decoupling, malonyl-CoA, secondary metabolism

Shengbiao Hu
contact affiliation	Hunan Normal University
contact email	shengbiaohu@hunnu.edu.cn
lab head
Zirui Dai
contact affiliation	Hunan Normal University
contact email	Daizr00035@hunnu.edu.cn
dataset submitter

iProX dataset URI