⮝ Full datasets listing
PXD080922-1
PXD080922 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | VRK1 phosphorylates SRRM2 for the regulation of splicing of DNA damage repair factors |
| Description | Vaccinia-related kinase 1 (VRK1) is a nuclear serine/threonine kinase that contributes to genome stability through the phosphorylation of diverse substrates. This study investigated whether VRK1 regulates pre-messenger RNA splicing through serine/arginine repetitive matrix protein 2 (SRRM2), a nuclear speckle-associated splicing scaffold. Messenger RNA sequencing was performed in control HEK293T cells, VRK1-knockout HEK293T cells, and SRRM2-depleted HEK293T cells. Alternative splicing events were analyzed using replicate Multivariate Analysis of Transcript Splicing. Most differential alternative splicing events detected following VRK1 knockout or SRRM2 depletion were skipped-exon events. A subset of skipped-exon events was shared between VRK1-knockout and SRRM2-depleted cells, and the corresponding genes were enriched in DNA repair-related pathways. These data support a role for the VRK1-SRRM2 regulatory axis in maintaining the efficient alternative splicing of DNA damage response-related transcripts. |
| HostingRepository | iProX |
| AnnounceDate | 2026-07-09 |
| AnnouncementXML | Submission_2026-07-13_01:01:18.742.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jinghan Rong |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | phosphorylated residue |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-13 01:00:08 | ID requested | |
| ⏵ 1 | 2026-07-13 01:01:19 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: VRK1, SRRM2, alternative splicing, phosphorylation, DNA damage response |
Contact List
| Xiaochun Yu | |
|---|---|
| contact affiliation | Westlake University |
| contact email | yuxiaochun@westlake.edu.cn |
| lab head | |
| Jinghan Rong | |
| contact affiliation | Westlake University |
| contact email | rongjinghan@westlake.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
