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PXD080824-1

PXD080824 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of MAST1 interactors in dorsal brain organoids
DescriptionMAST1 was N-terminally EGFP-tagged and overexpressed in HEK293 cells. Two constructs of MAST1, (WT and S90A S161A), as well as an EGFP only control, were compared in a pairwise manner to identify specific interactors of MAST1. EGFP-MAST1 was affinity-purified using the GFP-Trap system and then incubated with the lysate of dorsal brain organoids. The interacting proteins were analyzed by mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2026-09-02
AnnouncementXMLSubmission_2026-09-02_06:11:30.109.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarkus Hartl
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02026-07-09 14:03:56ID requested
12026-09-02 06:11:30announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: AP-MS,MAST1, brain organoid
Contact List
Thomas A. Leonard
contact affiliationMedical University of Vienna, Max Perutz Labs, Dr. Bohr-Gasse 9, 1030 Vienna, Austria
contact emailThomas.leonard@meduniwien.ac.at
lab head
Markus Hartl
contact affiliationMax Perutz Labs, Mass Spectrometry Facility, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna, Austria
contact emailproteomics@maxperutzlabs.ac.at
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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