PXD080731-1

PXD080731 is an original dataset announced via ProteomeXchange.

Title	Rapid and Efficient Isolation of Bioactive Extracellular Vesicles Using an Optimized Amine-Functionalized Anion-Exchange Membrane
Description	We developed an ion exchange cellulose membrane (IEM) functionalized with tertiary amine groups. The IEM, prepared by functionalizing a base membrane with tertiary amine groups to achieve an ion exchange capacity of 0.10–0.11 meq/g, is capable of processing approximately 4 × 109 EVs/cm². Using this membrane, EVs in culture supernatants or plasma can be rapidly separated and purified within 30 minutes, without a concentration step, into EXO-rich small EVs. Importantly, this method demonstrates high reproducibility across lots while preserving biological functions, including target cell specificity and systemic circulation.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-17_06:28:30.919.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naohiro Seo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-08 06:45:38	ID requested
⏵ 1	2026-08-17 06:28:31	announced

Dataset with its publication pending

submitter keyword: Surface electric charge, Zeta potential, Ion exchange membrane, Exosome,Extracellular vesicle, Small extracellular vesicle

Naohiro Seo
contact affiliation	Department of Bioengineering, The University of Tokyo
contact email	seo-naohiro@bionano.t.u-tokyo.ac.jp
lab head
Naohiro Seo
contact affiliation	Department of Bioengineering, The University of Tokyo
contact email	seo-naohiro@bionano.t.u-tokyo.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD080731

PRIDE project URI

• PRIDE
  1. PXD080731
     1. Label: PRIDE project
     2. Name: Rapid and Efficient Isolation of Bioactive Extracellular Vesicles Using an Optimized Amine-Functionalized Anion-Exchange Membrane