PXD080642 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Generation and Proteomic Analysis of Fibrin-Based 3D Engineered Vascular Tissues (EVTs) from Human Aortic Smooth Muscle Cells |
| Description | Vascular smooth muscle cells (SMCs) reside within the medial layer of blood vessels, where they interact with an extracellular matrix (ECM) composed of collagen, elastin, and proteoglycans to maintain vascular structure and function. Aberrant ECM remodeling contributes to multiple vascular diseases; however, conventional two-dimensional culture systems do not adequately recapitulate the three-dimensional (3D) cellular and matrix environment required to study smooth muscle cell–ECM interactions and matrix remodeling. This protocol describes the generation of engineered vascular tissues (EVTs) from primary human aortic SMCs cultured within fibrin-based 3D hydrogels. Following casting between flexible polydimethylsiloxane posts, EVTs undergo cellular alignment, contraction, and deposition of de novo ECM, providing a physiologically relevant platform for studying vascular matrix biology. The protocol details tissue fabrication, culture, harvesting, and downstream analysis of newly deposited ECM. In addition, a workflow is presented for qualitative and quantitative characterization of EVT-derived proteins using Western blotting and mass spectrometry-based proteomics. Sequential protein extraction enables assessment of soluble and ECM-enriched protein fractions, facilitating in-depth evaluation of ECM composition. This platform provides a reproducible approach for investigating ECM production and remodeling by human SMCs in a 3D environment. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-24 |
| AnnouncementXML | Submission_2026-08-24_04:16:07.358.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | XIAOKE YIN |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; (18)O labeled deglycosylated asparagine; iodoacetamide derivatized residue |
| Instrument | Orbitrap Astral Zoom |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-07-06 23:36:19 | ID requested | |
| ⏵ 1 | 2026-08-24 04:16:07 | announced | |
Publication List
Keyword List
| submitter keyword: Tissue engineering |
| 3D cell culture;Smooth muscle cells |
| Extracellular matrix |
| Cardiovascular disease |
Contact List
| Manuel Mayr |
|---|
| contact affiliation | National Heart and Lung Institute, Imperial College London, London, UK |
| contact email | m.mayr@imperial.ac.uk |
| lab head | |
| XIAOKE YIN |
|---|
| contact affiliation | National Heart and Lung Institute, Faculty of Medicine, Imperial College London |
| contact email | xiaoke.yin@imperial.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD080642
- Label: PRIDE project
- Name: Generation and Proteomic Analysis of Fibrin-Based 3D Engineered Vascular Tissues (EVTs) from Human Aortic Smooth Muscle Cells
