PXD080543-1

PXD080543 is an original dataset announced via ProteomeXchange.

Title	Crosstalk between S-nitrosylation and glycation defines a novel metabolic vulnerability in liver and renal cancers
Description	Methylglyoxal (MGO)-derived advanced glycation end products (AGEs) are hallmarks of carbonyl stress and contribute to cancer progression. Here, we established and validated an LC–MS/MS workflow to detect MGO-induced protein modifications in wild-type (WT) and AKR1A1 knockout (KO) Huh7 cells. Following MGO treatment, proteins were digested and analyzed by liquid chromatography–tandem mass spectrometry to identify characteristic glycation products, including methylglyoxal-derived hydroimidazolone (MG-H1) and Nε-(1-carboxyethyl)-L-lysine (CEL). Comparative analysis demonstrated increased accumulation of MGO-derived modifications in AKR1A1 KO cells compared with WT cells, consistent with impaired detoxification of reactive dicarbonyl species. These findings validate a robust LC–MS/MS workflow for profiling MGO-mediated protein glycation and support its application for investigating carbonyl stress in cancer models.
HostingRepository	PRIDE
AnnounceDate	2026-07-08
AnnouncementXML	Submission_2026-07-08_11:28:40.635.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcus Nielsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-03 07:55:54	ID requested
⏵ 1	2026-07-08 11:28:41	announced

Dataset with its publication pending

submitter keyword: Human, QE-Plus

Marcus Nielsen
contact affiliation	Roskilde University, Department of Science and Environment, Denmark
contact email	marcusn@ruc.dk
lab head
Marcus Nielsen
contact affiliation	Roskilde university
contact email	marcusn@ruc.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD080543
     1. Label: PRIDE project
     2. Name: Crosstalk between S-nitrosylation and glycation defines a novel metabolic vulnerability in liver and renal cancers