PXD080453-1

PXD080453 is an original dataset announced via ProteomeXchange.

Title	Luteolibacter sp. VK1 comparative proteomics
Description	Bacterial exopolysaccharides (EPS) display extensive structural diversity arising from variation in monosaccharide composition, glycosidic linkages, and non-carbohydrate modifications. However, the enzymes responsible for degrading many EPS structures remain unknown, reflecting the limited coverage of current carbohydrate-active enzyme (CAZyme) families. Here, we investigated the microbial degradation of EPSLp, a high-molecular-mass glycosaminogalactan from Lactiplantibacillus pentosus KW1 containing the uncommon GalNAcβ1→2(4,6-O-R-pyruvyl)Gal motif. Enrichment cultivation with EPSLp as the sole carbon source led to the isolation of Luteolibacter sp. VK1, a novel strain capable of utilizing this biopolymer. Size-exclusion chromatography and MALDI-TOF mass spectrometry showed that VK1 depolymerizes EPSLp into defined oligosaccharides, consistent with endo-acting cleavage followed by further processing. By combining genomics, AlphaFold-based structural annotation and comparative proteomics, we identified candidate CAZymes potentially involved in EPSLp catabolism. Notably, a secreted GH123 protein and a distantly related GH123-like protein were strongly associated with EPSLp-dependent growth, suggesting that this enzyme family, or related structural homologues, may contribute to extracellular EPSLp depolymerization. Together, these findings reveal a candidate pathway for degradation of a structurally complex, pyruvylated bacterial glycan and provide a foundation for biochemical characterization of novel EPS-active enzymes.
HostingRepository	PRIDE
AnnounceDate	2026-09-04
AnnouncementXML	Submission_2026-09-04_08:28:59.568.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victor Daisuke Kietzmann
SpeciesList	scientific name: Luteolibacter; NCBI TaxID: NEWT:518753;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-01 16:03:12	ID requested
⏵ 1	2026-09-04 08:29:00	announced

Dataset with its publication pending

submitter keyword: Exopolysaccharide, Q-Exactive,Luteolibacter, Proteomics

Vincent Eijsink
contact affiliation	Protein Engineering and Proteomics Group, Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences
contact email	vincent.eijsink@nmbu.no
lab head
Victor Daisuke Kietzmann
contact affiliation	Norwegian University of Life Sciences
contact email	victor.daisuke.kietzmann@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD080453
     1. Label: PRIDE project
     2. Name: Luteolibacter sp. VK1 comparative proteomics