PXD080395-1

PXD080395 is an original dataset announced via ProteomeXchange.

Title	Immunoprecipitation–mass spectrometry analysis of VafA/VafB-binding proteins in Escherichia coli
Description	FLAG-tagged VafA and VafB were expressed in E. coli BL21(DE3) cells, and VafA/VafB-associated proteins were immunoprecipitated from cell lysates using anti-DYKDDDDK magnetic beads. Bead-bound proteins were digested on beads with trypsin/Lys-C mix, and the resulting peptides were desalted and analyzed by LC-MS/MS using an EASY-nLC 1200 system coupled to an Orbitrap Fusion mass spectrometer. Raw data were searched against the SwissProt E. coli database supplemented with VafA and VafB sequences using Proteome Discoverer 2.5. Peptides were filtered at a 1% false discovery rate using the Percolator node, and label-free precursor ion quantification was performed.
HostingRepository	jPOST
AnnounceDate	2026-07-01
AnnouncementXML	Submission_2026-06-30_18:25:40.735.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidetaka Kosako
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	L-methionine sulfoxide; Acetyl; S-carboxamidomethyl-L-cysteine
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-30 18:25:18	ID requested
⏵ 1	2026-06-30 18:25:41	announced

Dataset with its publication pending

submitter keyword: IP-MS, VafA, VafB, E. coli

Toyotaka Sato
lab head
Hidetaka Kosako
contact affiliation	Tokushima University
dataset submitter

jPOST dataset URI

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