PXD080393-1

PXD080393 is an original dataset announced via ProteomeXchange.

Title	Plasma proteomics across the first 48 hours of life: High-resolution analysis of 906 peripheral blood samples from 679 healthy term neonates in The Gambia
Description	Early postnatal life involves rapid physiological and immunological adaptation as the newborn transitions from intrauterine to extrauterine conditions. These processes are reflected in the circulating plasma proteome and affect key blood functions, including homeostasis, hemostasis, and immune defense. During pregnancy, immune tolerance is tightly regulated within the largely sterile intrauterine environment. After birth, the neonatal immune system undergoes rapid maturation to balance antimicrobial defense with colonization by commensal microbes. This early-life immune development, primarily driven by innate immune mechanisms and maternally derived antibodies, has long-term implications for health and disease and exhibits sex-specific characteristics. To characterize early plasma proteome dynamics, this study leverages a large, well-controlled cohort of 679 healthy term newborns enrolled in The Gambia as part of the NIH/NIAID Human Immunology Project Consortium (HIPC). Peripheral blood samples were obtained from all participants within the first 24 hours of life (Day of Life 0), with additional samples collected from a subset on Day of Life 1, resulting in 906 plasma samples spanning the first 48 hours after birth. Using a high-throughput targeted mass spectrometry approach, plasma proteins were quantified at bi-hourly resolution. The dataset provides a normative, high-resolution resource for studying early-life proteome dynamics and supports future mechanistic and functional investigations in human neonatal biology.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-17_07:02:51.739.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LCMS-8060NX

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-30 14:34:36	ID requested
⏵ 1	2026-08-17 07:02:52	announced

Dataset with its publication pending

submitter keyword: Proteomics

neonatal biology

plasma

MRM

targeted mass spectrometry

ontogeny

early life immunity

Hanno Steen
contact affiliation	Boston Children's Hospital
contact email	hanno.steen@childrens.harvard.edu
lab head
Jing Chen
contact affiliation	Boston Children's Hospital
contact email	jing.chen2@childrens.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD080393

PRIDE project URI

• PRIDE
  1. PXD080393
     1. Label: PRIDE project
     2. Name: Plasma proteomics across the first 48 hours of life: High-resolution analysis of 906 peripheral blood samples from 679 healthy term neonates in The Gambia