PXD080319-1

PXD080319 is an original dataset announced via ProteomeXchange.

Title	Polo/PLK1 phosphorylation relieves Centrosomin (Cnn) autoinhibition to drive centrosome scaffold assembly
Description	Mitotic centrosome maturation requires Polo/PLK1-dependent expansion of the pericentriolar material (PCM). In Drosophila, Centrosomin (Cnn) assembles a scaffold around mitotic centrioles through interactions between its PReM and CM2 domains. Here, we show that PReM adopts an autoinhibited helical hairpin conformation that prevents CM2 binding. Polo/PLK1 phosphorylation relieves this autoinhibition, enabling scaffold assembly, whereas phospho-blocking mutations disrupt PReM–CM2 binding in vitro and Cnn scaffold assembly in vivo. Potential functionally analogous domains have been identified in the human and C. elegans Cnn homologues CDK5RAP2 and SPD-5. We find that the human protein appears to share a structurally similar mechanism for scaffold assembly, but the worm protein does not. Consistent with this, deletion of these domains alters the dynamics of Cnn condensates in vitro, but has little effect on SPD-5 condensate dynamics. We conclude that Polo/PLK1 promotes mitotic centrosome assembly, at least in part, by relieving autoinhibitory intramolecular interactions.
HostingRepository	PRIDE
AnnounceDate	2026-07-23
AnnouncementXML	Submission_2026-07-23_08:11:20.220.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marjorie Fournier
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral Zoom; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-29 03:31:33	ID requested
⏵ 1	2026-07-23 08:11:20	announced

Dataset with its publication pending

submitter keyword: Centrosomin,PLK1, centrosome scaffold assembly

Jordan Raff
contact affiliation	Sir William Dunn School of Biology, University of Oxford
contact email	jordan.raff@path.ox.ac.uk
lab head
Marjorie Fournier
contact affiliation	University of Oxford
contact email	marjorie.fournier@bioch.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD080319
     1. Label: PRIDE project
     2. Name: Polo/PLK1 phosphorylation relieves Centrosomin (Cnn) autoinhibition to drive centrosome scaffold assembly