⮝ Full datasets listing
PXD080310-1
PXD080310 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cardiac Lysine Succinylome Profiling of Wild-Type and SIRT5-Deficient Mice at 4 Months after Irradiation |
| Description | This study aimed to investigate the role of SIRT5 in regulating the cardiac succinylome following radiation exposure. The experiment utilized a cohort of six mice, comprising three SIRT5-knockout (SIRT5-KO) and three wild-type (WT) mice. All subjects received a single dose of thoracic radiation. Four months post-irradiation, cardiac tissues were collected. Quantitative proteomic analysis of succinylation modifications was then performed. Specifically, proteins were extracted from the heart tissues, digested with trypsin, and the resulting peptides were subjected to immunoaffinity enrichment using a high-specificity anti-succinyllysine antibody. The enriched succinylated peptides were analyzed using a timsTOF mass spectrometer coupled with nano-liquid chromatography in a 4D-data-independent acquisition (4D-FastDIA) mode. This advanced method provided high-depth, quantitative profiling of succinylation sites. The raw data were processed to identify and quantify lysine succinylation sites, enabling a comparative |
| HostingRepository | iProX |
| AnnounceDate | 2026-06-29 |
| AnnouncementXML | Submission_2026-06-29_02:21:00.187.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhiming Zeng |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | N6-succinyl-L-lysine |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-29 02:19:49 | ID requested | |
| ⏵ 1 | 2026-06-29 02:21:00 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SIRT5, lysine succinylation, succinylome, radiation-induced heart disease |
Contact List
| Anwen Liu | |
|---|---|
| contact affiliation | The Second Affiliated Hospital of Nanchang University |
| contact email | awliu666@163.com |
| lab head | |
| Zhiming Zeng | |
| contact affiliation | The Second Affiliated Hospital of Nanchang University |
| contact email | 2zm@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
