PXD080281-1

PXD080281 is an original dataset announced via ProteomeXchange.

Title	Integrating Sucrose Density Gradient Centrifugation with Microscale Proteomics to Screen for Magnesium-Dependent Phase Separation Candidates
Description	his dataset examines the proteomic remodeling of phase-separated protein condensates in response to high-magnesium stimulation, utilizing discontinuous sucrose density gradient ultracentrifugation combined with ultra-low input mass spectrometry. RIPA cell lysates were layered onto a stepwise sucrose gradient (10%, 22.5%, and 35% from bottom to top) and centrifuged at 250,000 g for 4 hours at 4°C. Three fractions (top, middle, and bottom 35% dense layer) were collected post-centrifugation. To identify putative phase-separation candidates, we compared protein distribution in every density bottom fraction between human PASMC cells with low endogenous UBXN1 expression and human cells overexpressing UBXN1 under high-magnesium treatment (24–48 h).
HostingRepository	iProX
AnnounceDate	2026-06-27
AnnouncementXML	Submission_2026-06-29_01:13:15.080.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiayu Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-29 01:11:35	ID requested
⏵ 1	2026-06-29 01:13:15	announced

Dataset with its publication pending

submitter keyword: Pulmonary Arteries

Jiayu Liu
contact affiliation	Fujian Medical University
contact email	jiayuliu@fjmu.edu.cn
lab head
Jiayu Liu
contact affiliation	Fujian Medical University
contact email	jiayuliu@fjmu.edu.cn
dataset submitter

iProX dataset URI