⮝ Full datasets listing
PXD080243-1
PXD080243 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The 4EHP/GIGYF2 translation repressor complex is co-opted by the Vaccinia Virus K3L protein as a potential mechanism of immunoevasion |
| Description | Poxviruses have evolved robust immunoevasion strategies that enable efficient replication in the cytosol. We previously reported that, in addition to several RNA viruses, vaccinia virus replication is impaired in the absence of the host cap-binding translation repressor protein 4EHP/eIF4E2, implicating it in host antiviral defense and poxvirus-mediated immunosuppression. Here, we show that the vaccinia virus-encoded K3L protein interacts with 4EHP via its known binding partner GIGYF2 to increase the stability of the 4EHP/GIGYF2 complex. Consequently, K3L bolsters the 4EHP/GIGYF2-mediated mRNA translation repression. Thus, we demonstrate a 4EHP/GIGYF2-dependent mechanism conserved between RNA and DNA viruses that likely mediates immunoevasion. Our findings also document a 4EHP/GIGYF2-dependent stringent regulation of infection by poxviruses, a clinically important family of viruses that are used as vaccine vectors and in oncolytic viral therapy. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-21 |
| AnnouncementXML | Submission_2026-08-21_07:24:59.014.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Adrian Pelin |
| SpeciesList | scientific name: Vaccinia virus; NCBI TaxID: 10245; scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-26 15:58:45 | ID requested | |
| ⏵ 1 | 2026-08-21 07:24:59 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: vaccinia, K3L, DatasetType:Proteomics |
Contact List
| Dr. Nevan J. Krogan | |
|---|---|
| contact affiliation | Department of Cellular and Molecular Pharmacology, University of California San Francisco, USA |
| contact email | nevan.krogan@ucsf.edu |
| lab head | |
| Adrian Pelin | |
| contact affiliation | UCSF |
| contact email | apelin20@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v13/MSV000102289/ |
