PXD080233 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phage depolymerase modulates virulence and biofilm in hypervirulent Klebsiella pneumoniae by CPS-mediated suppression of type 3 fimbriae |
| Description | Phage-derived depolymerases represent a promising antibiotic alternative for treating Klebsiella pneumoniae infections. Depolymerases can increase the sensitivity of bacteria to the host immune system and complement-mediated killing by specifically degrading capsular polysaccharides (CPS). However, the impact of depolymerases on bacterial biofilm formation remains unclear. This study found that depolymerase treatment significantly enhances the biofilm formation capability of hypervirulent K. pneumoniae (hvKp) strains, which inherently exhibit relatively weak biofilm formation due to their thick capsular polysaccharide (CPS) layers. Further investigation revealed that depolymerase-mediated CPS degradation relieved its repression on the type 3 fimbriae gene cluster mrkABCDF, thereby promoting biofilm formation. In vivo experiments in mice also showed that CPS can inhibit virulence functions associated with type 3 fimbriae. Furthermore, we found that CPS-mediated biofilm inhibition appears to be a common phenomenon among hvKp strains. In summary, by elucidating the dual role of depolymerase in modulating both virulence and biofilm in hvKp, our work reveals a potential interaction between CPS and type 3 fimbriae, providing deeper insights into the pathogenicity of this clinically important bacterium. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-28 |
| AnnouncementXML | Submission_2026-06-28_00:34:00.258.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuchen Chen |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Klebsiella pneumoniae; NCBI TaxID: NEWT:573; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-06-26 09:45:31 | ID requested | |
| ⏵ 1 | 2026-06-28 00:34:00 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Klebsiella pneumoniae |
| Capsular polysaccharides |
| Type 3 fimbriae |
| Depolymerase |
| Virulence |
| Biofilm |
Contact List
| Yuchen Chen |
|---|
| contact affiliation | NO.2 Yabao Road, Beijing Province |
| contact email | cyc15261284562@qq.com |
| lab head | |
| Yuchen Chen |
|---|
| contact affiliation | Capital Institute of Pediatrics |
| contact email | cyc15261284562@qq.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD080233
- Label: PRIDE project
- Name: Phage depolymerase modulates virulence and biofilm in hypervirulent Klebsiella pneumoniae by CPS-mediated suppression of type 3 fimbriae
