PXD080181-1

PXD080181 is an original dataset announced via ProteomeXchange.

Title	Platycodi Radix Extract Prevents Hepatic Steatosis by Enhancing Bile Acid Synthesis in a High-Fat Diet-Induced Fatty Liver Mouse Model
Description	This project investigated the molecular mechanism by which *Platycodi Radix* extract (PRE; the ethanol extract of the root of *Platycodon grandiflorum*) prevents non-alcoholic fatty liver disease (NAFLD) in a high-fat diet (HFD)-induced fatty liver mouse model, using a liver-tissue shotgun proteomic and bioinformatic approach. Five-week-old male C57BL/6J mice were acclimatized and then maintained for 12 weeks on one of three diets: a normal chow diet (NCD), a high-fat diet (HFD), or an HFD supplemented with 5% (w/w) PRE. PRE supplementation prevented HFD-induced body-weight gain, fat accumulation, and hepatic steatosis, and improved glucose tolerance and serum lipid profiles. To elucidate the underlying mechanism, liver proteomes of the NCD, HFD, and PRE groups were profiled by LC-MS/MS (n = 3 per group), and proteins differing in relative abundance between NCD vs HFD and HFD vs PRE were interpreted by Ingenuity Pathway Analysis. This LC-MS/MS dataset was designed as a discovery-stage, exploratory screen for hypothesis generation rather than as standalone quantitative proof of differential expression; the resulting candidate proteins and pathways (notably decreased FXR/RXR-activation proteins and increased cholesterol-biosynthesis proteins, predicting enhanced bile-acid synthesis) were subsequently validated by targeted Western blotting and biochemical bile-acid measurement, on which the mechanistic conclusions of the study are based. This dataset comprises the liver-tissue LC-MS/MS data and the protein identification/quantification results supporting the conclusion that PRE prevents hepatic steatosis through modulation of FXR/RXR activation, cholesterol biosynthesis, and bile-acid synthesis pathways.
HostingRepository	PRIDE
AnnounceDate	2026-07-04
AnnouncementXML	Submission_2026-07-04_01:38:52.011.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wooyoung Kim
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-25 09:12:53	ID requested
⏵ 1	2026-07-04 01:38:53	announced

10.3390/nu16060893;

Kim W, Baek WH, Yun SH, Lee H, Kim MJ, Lee SY, Kim GH, Kim SI, Jeong HG, Park EC, Extract Prevents Hepatic Steatosis by Enhancing Bile Acid Synthesis in a High-Fat Diet-Induced Fatty Liver Mouse Model. Nutrients, 16(6):(2024) [pubmed]

ProteomeXchange project tag: Liver (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Food and Nutrition (B/D-HPP), Human Proteome Project

submitter keyword: Platycodon Radix,Proteomics, Obesity, NAFLD

Edmond Changkyun Park
contact affiliation	Korean Basic Science Instittue
contact email	edpark@kbsi.re.kr
lab head
Wooyoung Kim
contact affiliation	Korea Basic Science Institute
contact email	wykim91@kbsi.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080181

PRIDE project URI

• PRIDE
  1. PXD080181
     1. Label: PRIDE project
     2. Name: Platycodi Radix Extract Prevents Hepatic Steatosis by Enhancing Bile Acid Synthesis in a High-Fat Diet-Induced Fatty Liver Mouse Model