PXD080115-2

PXD080115 is an original dataset announced via ProteomeXchange.

Title	Quantitative SWATH Proteomic Analysis of Campylobacter jejuni and Campylobacter coli Following Exposure to the Antimicrobial Ionophore PBT2
Description	Campylobacter jejuni ATCC 33560 and Campylobacter coli ATCC BAA-1061 were exposed to the antimicrobial ionophore PBT2 to investigate proteome-wide responses associated with its antibacterial activity. Cultures were grown in Mueller-Hinton broth supplemented with 5% defibrinated horse blood and harvested at defined time points following treatment with PBT2 or untreated control conditions. Quantitative proteomic profiling was performed using SWATH data-independent acquisition (DIA) mass spectrometry. The resulting dataset provides a comprehensive characterization of protein abundance changes associated with PBT2 exposure, including alterations in pathways related to translation, respiratory metabolism, cell envelope integrity, motility, and metal homeostasis. These data provide insights into the molecular mechanisms underlying the antimicrobial activity of PBT2 against clinically relevant Campylobacter species.
HostingRepository	PRIDE
AnnounceDate	2026-07-24
AnnouncementXML	Submission_2026-07-24_06:51:49.752.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huiting Chen
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni ATCC 33560; NCBI TaxID: NEWT:889247; scientific name: Campylobacter coli RM2228; NCBI TaxID: NEWT:306254;
ModificationList	No PTMs are included in the dataset
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-24 05:36:43	ID requested
1	2026-06-26 22:26:52	announced
⏵ 2	2026-07-24 06:51:50	announced	2026-07-24: Updated project metadata.

Dataset with its publication pending

submitter keyword: Campylobacter coli, ionophore, PBT2, SWATH-MS,Campylobacter jejuni

Freda Jen
contact affiliation	Institute for Biomedicine and Glycomics, Griffith University, Gold Coast
contact email	f.jen@griffith.edu.au
lab head
Huiting Chen
contact affiliation	Institue for Biomedicine and Glycomics
contact email	huiting.chen2@griffithuni.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD080115

PRIDE project URI

• PRIDE
  1. PXD080115
     1. Label: PRIDE project
     2. Name: Quantitative SWATH Proteomic Analysis of Campylobacter jejuni and Campylobacter coli Following Exposure to the Antimicrobial Ionophore PBT2