PXD080104-1

PXD080104 is an original dataset announced via ProteomeXchange.

Title	Mechanistic study of SENP2-PCYT1A axis regulating synaptic pruning that results in anxiety-like behavior
Description	Anxiety disorder, one of the most common mental illnesses, is emerging as a significant public health challenge. Glial regulation of neuronal synaptic pruning offers a novel therapeutic avenue for anxiety; however, it remains unclear whether SUMO modification is involved in this regulatory process. In this study, we demonstrate that both global and microglia-specific SENP2 knockout mice exhibit anxiety-like behaviors coupled with aberrant synaptic pruning, whereas neuronal-specific knockout animals do not show these phenotypes. Moreover, SENP2 specifically modulates LC3-associated phagocytosis (LAP) in microglia and removes SUMO modifications from the target protein PCYT1A. We propose that the SENP2–PCYT1A signaling axis in microglia/astrocyte governs LAP pathway activity, thereby maintaining synaptic pruning homeostasis and preventing the onset of anxiety-like behaviors. Accordingly, this project will (1) define the precise role of LAP in microglial/astrocyte synaptic pruning, (2) elucidate the molecular mechanism by which SUMOylation regulates key factors in the LAP pathway, and (3) investigate how the SENP2–PCYT1A axis modulates synaptic stability and anxiety-related behaviors. These findings not only advance our understanding of SUMO modification in microglia–neuron immune crosstalk but also provide novel insights into potential targeted interventions and precision therapies for anxiety-related mental disorders.
HostingRepository	iProX
AnnounceDate	2026-06-23
AnnouncementXML	Submission_2026-06-29_00:14:33.567.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meng Liu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-24 01:32:43	ID requested
⏵ 1	2026-06-29 00:14:34	announced

Dataset with its publication pending

submitter keyword: SUMOylation, Synaptic pruning, LC3 associated phagocytosis, Microglia, Anxiety-like behavior

Yitao Qi
contact affiliation	Shaanxi Normal University
contact email	qiyitao@snnu.edu.cn
lab head
Meng Liu
contact affiliation	Shaanxi Normal University
contact email	2872497619@qq.com
dataset submitter

iProX dataset URI