⮝ Full datasets listing
PXD080067-2
PXD080067 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Whole-proteome profiling of human lung cancer brain metastasis-associated fibroblasts |
| Description | Cancer-associated fibroblasts (CAFs) are increasingly recognized as an important component of the brain metastasis tumour microenvironment; however, their proteomic phenotype remains poorly characterized. This dataset contains mass spectrometry-based proteomic profiles of primary human brain metastasis-associated fibroblasts (BrM-CAFs) isolated from lung cancer brain metastases (L-BrM-CAF, n = 3) and control, non-tumorous subgalear fibroblasts (Subgalear-NF, n = 3). Whole-cell lysates were analyzed using data-independent acquisition (DIA) mass spectrometry, supported by a project-specific spectral library generated from data-dependent acquisition (DDA) measurements. The dataset was generated to characterize the molecular phenotype of BrM-CAFs and to investigate protein-level alterations associated with stromal activation, extracellular matrix remodelling, and tumour–stroma interactions. The data provide a resource for studies of the brain metastasis microenvironment and CAF biology. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-08 |
| AnnouncementXML | Submission_2026-08-08_14:36:16.985.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jakub Cervenka |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-23 08:43:42 | ID requested | |
| 1 | 2026-08-03 03:02:19 | announced | |
| ⏵ 2 | 2026-08-08 14:36:17 | announced | 2026-08-08: Updated project metadata. |
Publication List
| V, ý, molov, á B, Pfeiferov, á L, Smetana TK, V, ý, mola P, Ternerov, á N, Zuba, ľ M, Kol, á, ř M, Š, á, chov, á J, Gy, ö, ny, ö, rov, á S, Bal, á, ž, iov, á E, Houdov, á, Megov, á M, Č, ervenka J, Kupcov, á, Skaln, í, kov, á H, Š, panko M, Smetana K, Lacina L, Netuka D, Tom, á, š R, Bu, š, ek P, Š, edo A, Brain metastasis-associated fibroblasts shape the tumour microenvironment to enhance cancer cell invasion. Brain Pathol, ():e70129(2026) [pubmed] |
| 10.1111/bpa.70129; |
Keyword List
| submitter keyword: primary cell culture, cell migration, LC-MS,brain metastases, cancer-associated fibroblasts, extracellular matrix |
Contact List
| Helena Kupcová Skalníková | |
|---|---|
| contact affiliation | Laboratory of Proteomics, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, U Nemocnice 5, Prague 2, 128 00, Czech Republic |
| contact email | helena.skalnikova@lf1.cuni.cz |
| lab head | |
| Jakub Cervenka | |
| contact affiliation | Laboratory of Proteomics, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic |
| contact email | jakub.cervenka@lf1.cuni.cz |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD080067 |
| PRIDE project URI |
Repository Record List
[ + ]
