PXD080059-1

PXD080059 is an original dataset announced via ProteomeXchange.

Title	Evaluation of N8-propargyl spermidine (Probe 1) as a substrate of deoxyhypusine synthase in cellulo
Description	Two complementary experiments were performed to evaluate whether N8-propargyl spermidine is a substrate of deoxyhypusine synthase in cells. HEK293 and mouse embryonic fibroblast (MEF) cells were incubated with either N8-propargyl spermidine (Probe 1) or spermidine, lysed, and subjected to CuAAC ligation with azido-PEG3-biotin. In the MEF experiment, biotinylated proteins were enriched on NeutrAvidin agarose resin, digested on-bead with trypsin, and, following TMT labeling and LC-MS/MS analysis, the data were processed in MaxQuant and analyzed for intensity enrichment in samples derived from cells treated with Probe 1 versus spermidine. In the HEK293 experiment, proteins were first digested with trypsin, after which biotinylated peptides were enriched on NeutrAvidin agarose resin and analyzed by LC-MS/MS. During MaxQuant processing, the peptide-level data were searched for Probe 1-derived hypusine and deoxyhypusine modifications carrying the biotinylated triazole tag, as well as for Probe 1-derived thiotriazole adducts on cysteine residues.
HostingRepository	PRIDE
AnnounceDate	2026-06-23
AnnouncementXML	Submission_2026-06-23_06:53:16.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-23 06:42:15	ID requested
⏵ 1	2026-06-23 06:53:16	announced

Dataset with its publication pending

submitter keyword: N8-propargyl spermidine, MEF, chemical probe, hypusination, HEK293

Remigiusz Serwa
contact affiliation	IMol, Polish Academy of Sciences, M. Flisa 6, 02-247 Warsaw, Poland
contact email	r.serwa@imol.edu.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD080059
     1. Label: PRIDE project
     2. Name: Evaluation of N8-propargyl spermidine (Probe 1) as a substrate of deoxyhypusine synthase in cellulo