PXD079956-1

PXD079956 is an original dataset announced via ProteomeXchange.

Title	SWIFT-GP: A Rapid and Automated platform for the Sensitive profiling of Site-Specific Glycans from Trace Samples
Description	Sensitive glycoproteomic profiling of limited clinical samples is often hindered by labor-intensive, multi-step sample preparation and the substantial sample loss inherent to manual workflows. In this study, we developed SWIFT-GP (Seamless Workflow for Intact Fast Trace-level GlycoProteomics), an integrated platform that combines an optimized rapid digestion protocol with an online three-column enrichment and separation system and a match-between-runs (MBR) strategy for the fast and sensitive profiling of intact glycopeptides. By utilizing an integrated valve-switching configuration that minimizes manual handling, the total time from live cells to collected glycopeptide MS spectra was reduced from over 27 hours to 3 hours. Further integration with the MBR strategy markedly expanded the identification of IGPs while effectively reducing the missing values inherent in low-input sample analysis. The performance of SWIFT-GP was demonstrated by processing trace serum samples and microscale cellular inputs. Using 1 µg serum protein as the starting material followed by rapid digestion and online enrichment, 986 intact glycopeptides (IGPs) were reproducibly quantified. Notably, over 1,700 IGPs can be recovered from 104 cancer cells. Overall, SWIFT-GP provides an efficient and sensitive solution for site-specific glycosylation profiling of restricted clinical specimens, supporting applications in high-throughput and trace-level glycoproteomic research.
HostingRepository	jPOST
AnnounceDate	2026-08-02
AnnouncementXML	Submission_2026-08-01_08:00:07.755.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Guo
SpeciesList	scientific name: 智人（人类）; NCBI TaxID: 9606;
ModificationList	unknown modification; L-methionine sulfoxide; alpha-amino acetylated residue; S-carboxamidomethyl-L-cysteine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-21 22:05:17	ID requested
⏵ 1	2026-08-01 08:00:08	announced

Dataset with its publication pending

submitter keyword: intact glycopeptides, sample preparation, online enrichment

Mingming Dong
lab head
Xin Guo
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
dataset submitter

jPOST dataset URI

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