PXD079944-1

PXD079944 is an original dataset announced via ProteomeXchange.

Title	Label-Free Proteomic Analysis of Alterations Induced by Wnt10b Downregulation in the Developing Mouse Cerebellum
Description	This dataset presents a high-resolution, label-free quantitative (LFQ) bottom-up proteomic analysis profiling the molecular rearrangements within the cerebellar tissue architecture following the knockdown of the wingless-type MMTV integration site family, member 10B (Wnt10b) ligand. Using organotypic slice cultures derived from the postnatal mouse cerebellum, this study captures systemic disruptions in cellular homeostasis, with a strong functional enrichment of differentially expressed proteins mapping directly to localized translational machinery at both pre-synaptic and post-synaptic densities. The dataset serves to bridge transcriptional disruptions in the YY1-Wnt10b signaling axis with precise downstream synaptic proteostasis failures driving ataxic phenotypes.
HostingRepository	PRIDE
AnnounceDate	2026-09-02
AnnouncementXML	Submission_2026-09-02_00:29:03.935.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas.C.N. LEUNG
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-20 23:33:10	ID requested
⏵ 1	2026-09-02 00:29:05	announced

10.1016/j.isci.2026.116975;

Lui YL, Dong X, Leung TCN, Li TH, Chan TF, Kwan KM, Loss of YY1 in Purkinje cells triggers cerebellar ataxia via Wnt10b-mediated impairment of dendrite development. iScience, 29(8):116975(2026) [pubmed]

submitter keyword: LC-MS/MS, Wnt10b, Cerebellum, LFQ, Mouse

Prof. KM Kwan
contact affiliation	School of Life Sciences, The Chinese University of Hong Kong, Hong Kong
contact email	kmkwan@cuhk.edu.hk
lab head
Thomas.C.N. LEUNG
contact affiliation	School of Life Sciences, The Chinese University of Hong Kong, Hong Kong
contact email	cnleung0509@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD079944

PRIDE project URI

• PRIDE
  1. PXD079944
     1. Label: PRIDE project
     2. Name: Label-Free Proteomic Analysis of Alterations Induced by Wnt10b Downregulation in the Developing Mouse Cerebellum