PXD079850-1

PXD079850 is an original dataset announced via ProteomeXchange.

Title	Integrated Proteomic and Phosphoproteomic Profiling Reveals Distinct Disease- and Age-Related Molecular Alterations in the Retina and Hippocampus of APP/PS1/MAPT Mice
Description	Background: Retinal abnormalities have emerged as promising noninvasive indicators of early Alzheimer’s disease (AD), yet the molecular relationship between retinal and hippocampal alterations remains insufficiently understood. Methods: The synchronized proteomic and phosphoproteomic profiling of the hippocampus and retina was performed in APP/PS1/MAPT transgenic mice. Differentially expressed proteins (DEPs), differentially phosphorylated peptides (DPPs), enriched pathways, and kinase perturbations were systematically analyzed to distinguish disease-related and age-related molecular patterns. Results: The retina displayed earlier and broader AD-related molecular alterations than the hippocampus. Retinal AD-related DEPs, DPPs and kinase perturbations were enriched in metabolic and molecular pathways, whereas hippocampal AD-related molecules were associated with synapse/axon/neuroactive ligand-related pathways. Two tissues shared a restricted set of dysregulated molecules, including RTN4, HDAC6 and PALS1_S84, Conclusion: This study establishes a synchronized dual-tissue, dual-omics framework for dissecting AD-related pathology and age-associated remodeling, providing new insight into eye-brain molecular crosstalk and offering a theoretical basis for retina-based, noninvasive early diagnosis of AD.
HostingRepository	iProX
AnnounceDate	2026-06-17
AnnouncementXML	Submission_2026-06-17_20:44:22.680.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zheng Zhao
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	phosphorylation without neutral loss
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-17 20:44:02	ID requested
⏵ 1	2026-06-17 20:44:23	announced

Dataset with its publication pending

submitter keyword: Alzheimer’s disease, retina, hippocampus, proteomics, phosphoproteomics, aging, kinase perturbation

Zheng Zhao
contact affiliation	Affiliated Kangning Hospital of Ningbo University (Ningbo Kangning Hospital)
contact email	zhaozheng201007@163.com
lab head
Zheng Zhao
contact affiliation	Affiliated Kangning Hospital of Ningbo University (Ningbo Kangning Hospital)
contact email	zhaozheng201007@163.com
dataset submitter

iProX dataset URI