PXD079682 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphoproteomics Reveals EPHA2 and EGFR Activation Following Wnt Pathway Inhibition in Wnt-Driven Tumours |
| Description | Wnt signalling represses multiple receptor tyrosine kinases (RTKs) in Wnt-high cancers. To identify RTKs responsible for MAPK activation following Wnt pathway inhibition, phosphotyrosine-targeted mass spectrometry was performed on xenograft tumour samples derived from HPAF-II and HCT116 cells. Tumours were treated with the PORCN inhibitor ETC-159 or generated from TCF7L2 knockout cells to achieve pharmacological or genetic suppression of Wnt signalling. Immunoaffinity enrichment of phosphotyrosine-containing peptides followed by LC-MS/MS analysis identified increased phosphorylation of EPHA2, EGFR and ERBB2 upon Wnt inhibition. Functional studies demonstrated that EGFR acts as a convergent mediator linking Wnt inhibition and EPHA2 loss to MAPK activation. These data provide insight into RTK signalling rewiring in Wnt-driven tumours and identify therapeutic vulnerabilities involving EGFR and Wnt pathway co-targeting. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-20 |
| AnnouncementXML | Submission_2026-08-20_03:22:46.645.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Changyuan Hu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-06-14 17:38:03 | ID requested | |
| ⏵ 1 | 2026-08-20 03:22:47 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Wnt Signalling, EPHA2,Phosphoproteomics, EGFR, Immunoprecipitation-Mass Spectrometry, LC-MS/MS |
Contact List
| David M. Virshup |
|---|
| contact affiliation | Program in Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore Department of Pediatrics, Duke University School of Medicine, Durham, NC, USA |
| contact email | david.virshup@duke.edu |
| lab head | |
| Changyuan Hu |
|---|
| contact affiliation | Monash University |
| contact email | changyuan.hu@monash.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD079682 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD079682
- Label: PRIDE project
- Name: Phosphoproteomics Reveals EPHA2 and EGFR Activation Following Wnt Pathway Inhibition in Wnt-Driven Tumours
