PXD079661-1

PXD079661 is an original dataset announced via ProteomeXchange.

Title	Edaravone Promotes Adaptive Proteomic Remodeling and Improves Behavioral Outcomes in Chronic Heat Stress–Induced Brain Injury
Description	Background: Heat stress is an increasing global health concern associated with high mortality and multi-organ dysfunction. The brain is particularly vulnerable, leading to behavioral impairments. At the molecular level, heat stress disrupts proteins involved in synaptic communication, mitochondrial function, and cellular homeostasis. Current treatments are mainly symptomatic, highlighting the need for targeted neuroprotective strategies. Edaravone, a potent free-radical scavenger with anti-inflammatory properties, has demonstrated neuroprotective effects in various neurological conditions. This study aimed to evaluate the neuroprotective effects of edaravone against heat stress–induced behavioral dysfunction and to investigate the underlying molecular mechanisms using proteomic analysis. Methodology: Male Wistar rats (n = 54) were assigned to four groups: control, heat stress (HS), edaravone (Edv), and heat stress plus edaravone (HS+Edv). Heat stress and treatment were administered for 12 weeks. Behavioral assessments, including radial arm water maze (RAWM), open field test (OFT), elevated plus maze (EPM), and tail suspension test (TST), were conducted. Proteomic analysis of the cortex, hippocampus, and cerebellum was performed using UHPLC–QTOF-MS. Result: Heat stress induced significant impairments in memory, learning, anxiety-like, and depressive-like behaviors. Proteomic analysis revealed region-specific alterations, with the cerebral cortex showing the most pronounced changes, particularly in pathways related to mitochondrial function, oxidative stress, synaptic signaling, and protein quality control. The HS+Edv group showed partial normalization of behavioral outcomes and modulation of key proteomic pathways, including improved mitochondrial function, reduced oxidative stress, and preservation of synaptic and cytoskeletal integrity. Conclusion: Edaravone attenuates heat stress–induced brain dysfunction by promoting adaptive proteomic remodeling rather than complete restoration.
HostingRepository	PRIDE
AnnounceDate	2026-06-16
AnnouncementXML	Submission_2026-06-16_03:26:55.415.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruba Zenati
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Bruker software

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-12 19:58:55	ID requested
⏵ 1	2026-06-16 03:26:55	announced
2	2026-06-16 04:30:38	announced	2026-06-16: Updated project metadata.

Dataset with its publication pending

submitter keyword: Brain

Heat stress

Proteomics

Edaravone

Behavioral Dysfunction.

Prof. Mohammad Semreen
contact affiliation	Department of Medicinal Chemistry, College of Pharmacy, University of Sharjah
contact email	msemreen@sharjah.ac.ae
lab head
Ruba Zenati
contact affiliation	College of Pharmacy, University of Sharjah
contact email	rubazenati94@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD079661
     1. Label: PRIDE project
     2. Name: Edaravone Promotes Adaptive Proteomic Remodeling and Improves Behavioral Outcomes in Chronic Heat Stress–Induced Brain Injury